STAIN TECHNIQUE 



33 



Combinations of Stains 



Double stains 

 Orange II . 



X 



Triple stains 



Orange II (5) and 

 Biebrich Scarlet (2) 



X 



Orange II (10) and 



Acid Fuchsine ( 1 ) X 



Quadruple stains 

 " Sun Yellow (10), 

 Orange II (5), and 

 Crocein Scarlet MOO (5) or 

 Biebrich Scarlet (2) or 

 Acid Fuchsine ( 1 ) ... X 



Counter stains 



(1) Aldehyde Green 



(2) Aniline Blue 



(3) Methyl Violet 



(4) New Methylene Blue 



(5) Niagara Sky Blue 



(6) Thionine 



(7) Victoria Blue B 



(1) Methyl Violet 



(2) New Methylene Blue 



(3) Niagara Sky Blue 



(4) Victoria Blue B 



(1) New Methylene Blue 



(2) Niagara Sky Blue 



(1) New Methylene Blue 



(2) Niagara Sky Blue 



(3) Aniline Blue 



161. Orange R is an acid dye, light 3 to 4; J gram in 100 ml. 

 of distilled water and 1 ml. of 1 per cent sulfuric acid. Stain sec- 

 tions of animal tissues in a mixture of two parts Sun Yellow, C. I. 

 No. 620, and one part Orange R for ten minutes, rinse in distilled 

 water, and counterstain in Erie Violet BW, C. I. No. 387, eight 

 minutes for orange, red, and violet. 



On sections of animal or fungous tissues use a mixture of Sun 

 Yellow and Orange R for 10 minutes ; counterstain in Niagara Sky 

 Blue, C. I. No. 520, for one or two minutes. This combination 

 stain does not give satisfactory colors on tissues taken from a fixa- 

 tive containing picric acid. On plant tissues, Orange R has a more 

 reddish shade than Orange II; the counterstains are: (1) Methyl 

 Violet, C. I. No. 680, (2) Aldehyde Green, C. I. No. 676a, (3) 



