STAIN TECHNIQUE 



37 



Combinations of Stains 



Double stains 

 Biebrich Scarlet 



X 



Triple stains 



Biebrich Scarlet (2) and 

 Orange II (5) X 



Biebrich Scarlet (2) and 

 Sun Yellow (10) X 



Quadruple stains 



Biebrich Scarlet (2), 



Orange II (5), and 



Sun Yellow (10) X 



Counter stains 



(1) Aldehyde Green 



(2) Aniline Blue 



(3) Methyl Violet 



(4) New Methylene Blue 



(5) Niagara Sky Blue 



(6) Victoria Blue B 



(1) Methyl Violet 



(2) New Methylene Blue 



(3) Niagara Sky Blue 



(1) New Methylene Blue 



(2) Niagara Sky Blue 



(1) New Methylene Blue 



(2) Niagara Sky Blue 



286. Crocein Scarlet 7 B is an acid dye, light 3 ; J gram in 100 

 ml. of distilled water and 1 ml. of 1 per cent sulfuric acid. This 

 stain has properties similar to those of Biebrich Scarlet except that 

 it is more fuchsia. It may be used on animal tissues instead of 

 Acid Fuchsine. On sections of animal tissues use one of the triple 

 stains. Place the material in a mixture of five parts Sun Yellow, 

 C. I. No. 620, and one part Crocein Scarlet 7B for five minutes; 

 the counterstains are: (1) Niagara Sky Blue, C. I. No. 520, one 

 minute, (2) Aniline Blue, C. I. No. 707, 30 seconds, (3) Direct 

 Green B, C. I. No. 593, two minutes, or (4) Erie Violet BW, C. I. 

 No. 387, eight minutes. 



On mature plant tissues use Crocein Scarlet 7B for 24 hours 

 and counterstain with (1) Methyl Violet, C. I. No. 680, three min- 

 utes, (2) Victoria Blue B, C. I. No. 729, ten minutes, (3) Niagara 

 Sky Blue five minutes, or (4) Aniline Blue five minutes. 



289. Tolyl Blue 5R is a mordant dye, light 3 ; J gram in 100 

 ml. of 1 per cent ammonium acetate. With potassium dichromate 

 as the mordant, tracheids, pollen, and fungous mycelia are blue in 



