48 W. H. EMIG 



676a. Aldehyde Green, Aniline Green, Benzaldehyde Green is 

 a basic dye, light 4 ; J gram in 95 ml. of distilled water, 5 ml. of 5 per 

 cent ammonium alum, and % ml. of acetic acid. As a counterstain, 

 use Aldehyde Green for 15 minutes after Biebrich Scarlet, C. I. 

 No. 280, Acid Fuchsine, C. I. No. 692, Crocein Scarlet MOO, C. I. 

 No. 252, or Orange II, C. I. No. 151. It is similar to Malachite 

 Green, C. I. No. 657, but on xylem and sclerenchyma the green is a 

 shade brighter. 



677. Basic Fuchsine, Aniline Red, Diamond Fuchsine, Ma- 

 genta, Roseine is a basic dye, light 2 ; \ gram in 95 ml. of distilled 

 water, 5 ml. of 5 per cent ammonium alum, and J ml. of acetic acid ; 

 warm the mixture until the dye dissolves. Although Basic Fuch- 

 sine is more or less fugitive on fabrics, it is comparatively fast on 

 paraffin sections. It is used in certain bacteriological media and in 

 the staining of bacteria. Vascular plant tissues are stained an 

 intense fuchsia to reddish violet in 15 minutes ; the best counterstain 

 is Fast Green FCF, C. I. No. 670. For a good triple stain on free- 

 hand sections use a mixture of nine parts Orange II, C. I. No. 151, 

 and one part Benzo Pure Blue, C. I. No. 520, for one hour, and after 

 a rinse in distilled water, counterstain with Basic Fuchsine one 

 minute. 



678. New Fuchsine, New Magenta is a dye similar to but more 

 soluble and more bluish red than Basic Fuchsine. 



679. Dahlia, Hoffmann Violet, Iodine Violet, Red Violet 5R is 

 a basic dye, light 2 to 3 ; 1/10 gram in 95 ml. of distilled water, 5 ml. 

 of 5 per cent ammonium alum, and J ml. of acetic acid. The dye 

 may be used in any of the procedures described under Methyl Violet, 

 C. I. No. 680. A fast pigment is obtained if the material is stained 

 for an hour and differentiated in 95 per cent alcohol. Depending 

 on the tissue, the color varies from a reddish violet to dark violet or 

 black. 



680. Methyl Violet B, 2B is a basic dye, light 2 to 3 ; 1/10 gram 

 in 95 ml. of distilled water, 5 ml. of 5 per cent ammonium alum, and 

 J ml. of acetic acid. On animal tissues, basic dyes have a limited 

 value ; nuclei and a few kinds of special glandular cells such as gob- 

 let cells of the intestine and mucous cells of the epidermis are colored 

 much darker than the nonspecialized cytoplasmic structures. 



