viii Contents 



chapter 11. Making Smears and Squashes 119 



Smears— Squashes. 



chapter 12. Making Sections 124 



Nature of the Process— Free Sections— Paraffin Sections— Frozen 

 Sections. 



chapter 13. Cleaning, Labeling, and Storing Slides 168 



Part Three: Specific Examples of Slide Making 



example 1. Preparation of a Wholemount of a Mite by the Method of Berlese 175 



example 2. Preparation of a Wholemount of Pectinatella Stained in Gren- 

 adier's Alcoholic Borax Carmine 178 



example 3. Preparation of a Wholemount of 33-hour Chick Embryo, Using 



the Alum Hematoxylin Stain of Delafield 183 



example 4. Preparation of a Wholemount of a Liver Fluke, Using the Car- 



malum Stain of Mayer 189 



example 5. Smear Preparation of Monocystis from the Seminal Vesicle of an 



Earthworm 193 



example 6. Smear Preparation of Human Blood Stained by the Method of 



Wright 196 



example 7. Staining a Bacterial Film with Crystal Violet by the Technique 



of Lillie 199 



example 8. Demonstration of Gram-positive Bacteria in Smear Preparation 



by the Method of Gram 202 



example 9. Demonstration of Tubercle Bacilli in Sputum by the Technique 



of Neelsen 204 



example 10. Preparation of a Squash of the Salivary Glands of Drosophila 



Stained in LaCours Acetic Orcein 207 



example 11. Preparation of a Transverse Section of a Root, Using the Acid 



Fuchsin-Iodine Green Technique of Chamberlain 210 



example 12. Preparation of a Transverse Section of the Stnall Intestine of the 



Frog Stained ivith Celestine Blue B-Eosine 214 



example 13. Preparation of a Transverse Section of a Stem of Aristolochia 



Stained by the Method of Johansen 222 



example 14. Demonstration of Spermatogenesis in the Rat Testis, Using the 



Iron Hematoxylin Stain of Heidenhain 227 



example 15. Preparation of a Transverse Section of the Tongue of a Rat, 



Using Celestine Blue B Followed by Picro Acid Fuchsin 232 



example 16. Preparation of a Transverse Section of Amphioxus, Using the 



Acid Fuchsin— Aniline Blue-Orange G Stain of Mallory 235 



