Smear Preparation of Monocystis 195 



3. Dilute the contents of the infected seminal vesicle with 0.8% sodium 

 chloride to the consistency of a thin cream. 



4. Make a smear of cream as described in Chapter 11. 



5. Lay each smear face down on glass rods in fixative for 3 to 4 min. 

 Wash and accumulate in a coplin jar of 70% alcohol. 



6. Stain and differentiate smears. Dry in air. Mount in balsam. 



