Crystal Violet-stained Bacterial Film 201 



SUMMARY 



1. Use a wire loop to make a small thin smear of a diluted bacterial 

 culture on a clean slide. 



2. Dry smear in air, then pass it two or three times through a flame. 



3. Place a drop of crystal violet stain on the smear, allow it to remain 

 for 30 sec, and wash off with a jet of water from a wash bottle. 



4. Dry smear without heat. 



