Double-stained Hand Section of Root 213 



class at any level, and generally they will be found much better for this 

 purpose than the complex quadruple-stained sections in which the cyto- 

 logical detail all too often tends to obscure the clarity of morphological 

 detail, the chief requirement in this type of teaching. 



SUMMARY 



1. Cut hand sections of plant material hardened and preserved in alco- 

 hol. Accumulate sections in water. 



2. Place sections in 1% sodium or potassium hypochlorite until cell con- 

 tents have been dissolved, leaving clean bleached skeleton. 



3. Wash in water, rinse in 1% acetic acid, and wash again in water. 



4. Stain 24 hr in equal parts 0.2% acid fuchsin and 0.2% iodine green. 



5. Differentiate sections individually in absolute alcohol containing 1% 

 acetic acid and 0.1% iodine. 



6. Transfer directly to terpineol and mount in balsam. 



