226 Specific Examples of Slide Making 



3. Dehydrate through the mixtures of water, alcohol, and tertiary 

 butyl alcohol given in Chapter 8. 



4. Accumulate the pieces in pure tertiary butyl alcohol and add wax 

 little by little. 



5. Transfer to oven and complete embedding process through two 

 changes of pure wax. 



6. Make block and cut sections in the manner described in Example 10. 



7. Mount individual sections or short lengths of ribbon on slides, dry, 

 dewax, and transfer to 70% alcohol through the usual reagents. 



8. Stain in Johansen's safranin from 1 to 3 days. 



9. Wash thoroughly in running water and stain in 1% methyl violet 2B 

 for 10 to 15 min. 



10. Rinse in water and differentiate 10 to 15 sec in the first differentiat- 

 ing solution. 



11. Stain in fast green FCF from 10 to 20 min. 



12. Rinse in second differentiating solution 5 to 10 sec and stain in 

 orange G 3 to 5 min. 



13. Rinse off orange G in third differentiating mixture, remove this 

 mixture with special dehydrating agent, rinse in xylene, and mount in 

 balsam. 



