240 Specific Examples of Slide Making 



removed from them. It is desirable, therefore, as soon as differentiation 

 is complete, to dehydrate them in absolute alcohol and then clear them 

 in at least three changes of xylene, so as to make sure that no alcohol 

 can be carried over into the mounting medium. 



SUMMARY 



1. Fix diplococcus-infected liver in Zenker's fixative. 



2. Cut 5- to 8-/x sections. Mount on slide and run down to water through 

 usual reagents. 



3. Stain 1 hr in 2.5% phloxine at 55° C. 



4. Rinse briefly and transfer to Mallory's methylene blue-azur for 5 

 to 20 min. 



5. Wash in water. Differentiate in resin alcohol. 



6. Wash thoroughly in absolute alcohol. 



7. Clear in two or three changes of xylene and mount in balsam. 



