246 



Index 



Carmalum, 86 



Carmine, for staining, liver fluke, 189-192 

 Pectinatella, 178-182 



stains, 85, 86 

 Carnoy and Lebrun's fixative, 73 

 Cartilage, staining, 92, 93, 97 

 Cedar oil, 129 



Celestine blue B, applied to, intestine, 

 214-221 

 tongue, 233-234 



stain, 87, 88 

 Cellosolve, as debydrant, 99 



for differentiating stains, 95 



as ingredient of stain, 88 



as narcotic, 78 

 Centering of mechanical stage, 38, 39 

 Chamberlain's stain, 210-213 

 Chick embryo, sections of, 241-244 



wholemounts of, 183-188 



good and bad, examples of, 184 

 Chitin, softening, 77, 176 

 Chloral hydrate, as ingredient of moun- 

 tant, 105 



as narcotic, 78, 112 



for softening chitin, 77 

 Choroform, clearing agent, 101 



in fixative, 73 



as narcotic, 112 

 Chromic acid, 72, 73 

 Chromosomes, 73, 86, 96, 207, 229-231 



squash preparations, good and bad, 208 

 Cladocera, 176 

 Cleaning, coverslips, 220 



prepared slides, 168 



slides before use, 119, 218 

 Clearing agents, 100-102 



flotation technique, 102, 181 



phenol, 101 



terpineol, 100 



for wholemounts, 114 



xylene, 101 

 Clove oil, for clearing, 100 



in staining technique, 95 

 Coelenterata, 78, 112 

 Collembola, 176 



Condenser (see Field condenser; Sub- 

 stage condensers) 

 Connective tissue, 92, 93 

 Contrast, of photographic materials, 43, 

 44 



in photomicrography, 56 



Coplin jar, 67 



Coverslips, applying to wholemounts, 

 115-117 



cleaning, 220 



placing on sections, 160 



specifications for, 64, 65 



supporting over thick objects, 110 

 "Craf" fixatives, 73 

 Crystal violet, Lillie's, 89 



applied to bacteria, 199-200 

 Cut film, for photomicrography, 55 



processing, 48, 49 

 Cutting, freehand sections, 125-128 



frozen sections, 164 



paraffin sections, 146-149 

 Cutting facet, 142, 143 

 Cytoplasm, preservation of, 70 



Dark slide, 55 

 Decalcification, 76, 77 

 Dehydration, with cellosolve, 99 



with dioxane, 99 



with ethyl alcohol, 98, 99 



of plant tissue, 223, 224 



principles of, 98 



technique of, 113, 215, 216 

 Delafield's hematoxylin, 83, 84, 93 



applied to chick embryo, 187 

 Development, of papers, 49-50 



of photographic negatives, 46, 47 



theory of, 41 

 Dichromate fixatives, 71, 72 

 Dioxane, 99, 106, 129 

 Diplococci in section, 238-239 

 Direct hematoxylin stains, 82-84 

 Direct image projection, 51, 52 

 Dispersion, 5 



Distomum (see Liver fluke) 

 Dobell's hematoxylin, 82 

 Drierite, 103 

 Drosophila, 87, 207 

 Dyes, types of, 79 



E.F. (equivalent focus), 12 



Egg, incubation of, 183 



Egg albumen as ribbon adhesive, 150 



Ehrlich's hematoxylin, 83 



applied to chick sections, 243 

 Elder pith, 126 



Embedding, technique of, 134-140 

 Embedding boxes, 137, 138 



