248 



Index 



Heat as immobilizing agent, 69 

 Heidenhain's fixative, 72 

 Heidenhain's hematoxylin, 80 



applied to rat testis, 227 

 Helly's fixative, 71 



applied to frog intestine, 214 

 Hematein, 82 



Hematoxylin applied, to chick sections, 

 242 



to chick wholemount, 187 



to monocystis, 194 



to testis, 227 

 Hematoxylin stains, 80-84 



bluing, 81-83 

 Hucker's crystal violet, 89 



applied to bacterial smear, 200 

 Hydra, squash of, 122 

 Hydrochloric acid for differentiating, 82 

 Hydrozoa, 70 



Illuminant, for freshman microscope, 21, 

 23 



for medical microscope, 25, 26 



requirements of, 19 



for research microscope, 31, 34 

 Illumination, Kohler, 27 

 Image, direct projection of, 51 



formation of, 3, 6 



reflex projection of, 52, 53 



split beam, 53, 54 

 Immersion lenses, reason for, 10, 11 



setting up, 30, 37, 38 

 Immobilizing agents, 69 

 Incubation of hen's eggs, 183 

 Index of refraction, 7 



as affecting resolution, 10, 11 



of mounting media, 104 

 Indirect carmine staining, 85, 86 

 Indirect hematoxylin staining, 84 

 Invertebrates, staining, 113 

 Iodine, Lugol's, 75 

 Iodine green, 212 

 "Ipso" camera, 53 

 Iris diaphragm, 7 



function of, 15 

 Iron alum as mordant, 81, 82, 87 

 Iron hematoxylin stains, 81 



technique of differentiating, 229 



Johansen's quadruple stain, 95, 96 

 applied to plant stem, 224, 225 



Johansen's safranin, 88, 95 

 Jurray's mixture, 77 



Knives, microtome, sharpening, 144 

 Kohler illumination, 27 

 Kolmer's fixative, 74 



Labeling slides, 118, 169 

 LaCour's orcein, 86 



applied to salivary-gland chromosomes, 

 208 

 Lactophenol for swelling dried plant 



specimens, 210 

 Lamp (see Light sources) 

 Latent image, 40 

 Lavdowsky's fixative, 71 



applied to plant stem, 222 

 Lebrun ( see Carnoy and Lebrun's fixa- 

 tive) 

 Leeches, 112 



Leeuwenhoek, Anton van, 3 

 Leica, 53 

 Lenoir's fluid, 76 

 Lens, aberrations of, 4-6 



Coddington, 7 



doublet, 7 



meniscus, 7 



microscope ( see Objective; Ocular, 

 etc.) 

 Light green, 93 

 Light sources, 19, 30 



centering, 28, 35 

 Lillie's crystal violet, 89 



applied to bacterial smear, 199 

 Lithium carbonate for removal of picric 



acid, 76, 227 

 Liver fluke, good and bad mounts of, 190 



preparation of wholemount, 189-192 

 LoBianco's fixative, 72 



McNamara, Murphy, and Gore's solution, 



77 

 Magenta, 89 



applied to bacteria, 204 

 Magnesium sulfate as narcotic, 78, 112 

 Magnification, 11, 12 

 Mallory's triple stains, 82, 94 



applied, to Amphioxus, 236 

 to Diplococci in liver, 238-240 

 Maxwell's embedding wax, 131 

 Mayer's albumen, 150 



