250 



Index 



Ocular, types of, 14 

 Oil blue N, 96 

 Oligochaetae, 112 

 Orange G, 92, 95 



applied, to Amphioxus, 236 

 to Aristolochia, 222 

 Orcein, 86 



applied to chromosomes, 207 

 Oribatid mites, 176 

 Ortho-Illuminator, 31, 34, 35 

 Osmic acid, 69 



applied to smears, 121 

 Ovens, embedding, 131-133 



Panchromatic material, 43 

 Papers, photographic, 44, 45 

 Paraffin sections, 128-162 



action of knife in cutting, 143 



attaching to slide, 151 



clearing, animal material for, 129 

 plant material for, 130 



cold impregnation, 133-134 



combining single into ribbons, 242 



cutting, 147-149 



defects appearing, after mounting, 161- 

 162 

 while cutting, 155-157 



dehydration of material for, 129, 130 



embedding, media for, 130, 131 

 technique of, 131-140 



flattening, 152, 153 



knives for, 140-145 



making blocks for, 135, 138-140 



microtomes for, 140, 141 



mounting blocks for cutting, 146, 147 



mounting ribbons of, 149-154 



outline of technique for, 63, 128 



placing coverslip on, 160 



preparing embedding boxes for, 134- 

 137 



rolling to slide, 154, 233 



selection of fixative for, 128 



sharpening knives for, 144, 145 



staining, 158-160, 219 

 Patay's stain, 93 

 Pectinatella, 178-182 

 Petroleum jelly for temporary whole- 

 mounts, 108 

 Petrunkewitsch's fixative, 75 



applied to rat tongue, 232 



Phenol, for clearing, 101 



as ingredient, of fixative, 75 

 of stain, 89 



for softening chitin, 77 



for swelling dried plant specimens, 210 

 Phloroglucinol, 76 

 Phloxine, 94 



applied to sections, 238 

 Phosphomolybdic acid, 93 

 Phosphotungstic acid, 92 

 Photographic dark room, 45, 46 

 Photographic exposure, 56, 57 

 Photographic filters, 55, 56 

 Photographic negatives (see Negatives) 

 Photographic prints, processing, 49, 50 



selection of paper for, 44, 45 

 Photography, principles of, 40, 41 

 Picric acid, for differentiating stains, 81 



as fixative, 74 



removal from tissues, 76 



as stain, 90, 91, 232 

 Pipette, definition of, 65, 66 

 Plant chromosomes, 73 

 Plant tissues, dehydrating and clearing, 

 130 



freehand sections of, 126, 127 



staining, 113 

 Platyhelminthes, 112 

 Polychaetae, 72, 112 

 Polyvinyl alcohol, 105 

 Ponceau 2R, 91, 93 

 Potassium hydroxide for softening chitin, 



176 

 Potassium permanganate for bleaching 



carmine, 191 

 Prism, 5 

 Prothallium, 86 

 Protozoa, staining, 82 



wholemounts, 111-112 

 temporary, 109 



Rabbit liver, 238-239 

 Ramsden's disc, 4 

 Rat, testis, 227-231 

 tongue, 232-234 

 Reflex image projection, 52, 53 

 Refraction, 5 

 Regaud's hematoxylin, 81 

 Resin for differentiating stain, 94, 239 

 Resinous mountants, 106, 107 



