Index 



251 



Resolution, 8-11 



control of, 16, 17 



of photographic negatives, 42 



table of, 12 

 Richards, O. W., 154 

 Ringer's solution, 122, 207 

 Roll film processing, 47, 48 

 Root, section of, 210-213 

 Rotifera, 69, 78 

 Rubber paraffin, 131 



Safranin, 88, 95 



applied, to bacteria, 203 

 to plant tissues, 224 

 Salicylic acid in balsam, 93 

 Schantz microtome, 140 

 Section, of Amphioxus, 235-237 



of intestine, 214-221 



of liver, 238-239 



of plant stem, 222-226 



of rat tongue, 232 



of root, 210-213 



of 72-hour chick, 241-244 

 Section lifter, 65, 66 

 Sections, diagram of technique for, 63 



standard planes for, 124 



technique (see Freehand sections; Fro- 

 zen sections; Paraffin sections) 

 Seeds, fixative for, 74 

 Setting up, freshman microscope, 22-24 



immersion lens, 30, 37 



medical microscope, 26-30 



research microscope, 34-38 

 Sharpening microtome knives, 144 

 Shrinkage in fixation, 70 

 Silge and Kuhne, 31 

 Skeletonizing plant tissues, 211 

 Skeletons, staining, 96, 97 

 Slides, cleaning, 119, 120 



jars for staining, 67 



specifications of, 64 

 Smears, of bacteria, 199-200 



of blood, 196-198 



diagram of technique for, 62 



fixing, 121 



of monocystis, 193-195 



staining, 122 



technique of preparing, 110, 119 



of tubercle bacilli, 204-206 

 Smith's stain, 91 



Sodium bicarbonate for "bluing" 



hematoxylin, 83 

 Sodium hypochlorite, for hydrolyzing 

 sputum, 204 



for skeletonizing plant tissues, 211 

 Sodium sulfate, in fixative, 71 



in photographic hardener, 47 

 Softening chitin, 176 

 Solvents, "universal," 99 

 Spectral sensitivity of photographic 



negatives, 42 

 Speed, of papers, 44 



of photographic films, 42 

 Spencer microtome, 141, 163 

 Sphagnum moss, collecting small animals 



from, 176 

 Spirit blue, 91 



Split-beam image, projection of, 53, 54 

 Sputum, bacteria from, 204 

 Squashes, diagram of technique for, 62 



freezing to coverslip, 209 



of salivary gland, 207-209 



stain for, 87 



technique of, 122, 123 

 Staining, with acid fuchsin, 90, 92 



with alizarin red S, 96, 97 



with azure II, 94, 238 



bacteria, 94 



blood, 92-94 



bone, 93, 96, 97 



with carmine, 85, 86, 178, 189 



cartilage, 92, 93, 97 



with celestine blue B, 87, 88, 214, 233 



chromosomes, 86, 96 



connective tissue, 92, 94 



with crystal violet, 89, 199 



with eosin, 90, 93, 122, 214 



with fast green, 90, 95, 224 



fats, 96, 97 



freehand sections, 127 



with hematoxylin, 80-84, 187, 194, 242 



indirect, 80 



keratin, 92 



with light green, 93 



with magenta, 89, 204 



with methyl blue, 92, 235 



with methyl violet, 95, 222 



with methylene blue, 94, 119, 238 



mordant, 79, 80 



muscular tissues, 90, 92 



nuclei, 80-84, 93, 94 



