252 



Index 



Staining, with oil blue N, 96 



with orange G, 92 



with orange II, 91, 222, 235 



with orcein, 86, 87, 207 



paraffin sections, 158-160 



parasitic fungi, 96 



with phloxine, 94, 238 



with picric acid, 90, 91, 232 



plant sections, 94, 95 



plasma, 89-91 



with Ponceau 2R, 91, 93 



protozoa, 82 



purpose of, 79 



with safranin, 88, 95, 203, 224 



skeletons, 96, 97 



small invertebrates, 113 



smears, 122 



solutions (see name [e.g., Wright's 

 stain] or ingredient [e.g., Eosin] ) 



with spirit blue, 91 



squashes, 86 



with sudan IV, 96 



with toluidine blue, 97 



vertebrate embryos, 113 



xylem, 84, 96 



yolky material, 91 

 Steam for fixing amebas, 111, 112 

 Stender dish, 66 

 Storage, of paraffin blocks, 140 



of slides, 170 

 Stropping microtome knives, 144 

 Substage condensers, 14-18 



centering, 34 

 Sudan IV, 96 

 Supporting media, for freehand sections, 



125, 126 

 for frozen sections, 164 

 Surface of photographic papers, 45 

 Syracuse watch glasses, 65, 66 



Terpineol for clearing, 100, 114 

 Tert butyl alcohol, for dehydrating plant 

 materials, 130, 223 

 in Johansen's stain, 95 

 Testis, 227 

 Thysanura, 176 



Toluene for clearing tissues, 101 

 Toluidine blue, 97 



Tongue, 232-234 

 Trichloroacetic acid in fixative, 74 

 Trinitrophenol ( see Picric acid ) 

 Trinocular microscope, 53 

 Triple stains, 92-94 



"Universal" solvents, 99 

 Uranyl acetate, 74 

 Urea, 74 

 Useful magnification, 12 



van Gieson's stain, 90 



applied to rat tongue, 233 

 van Wijhe's stain, 97 

 Vials, 66 

 Vortex, 112 



Washing photographic negatives, 47 

 Watch glasses, 65, 66 

 Wavelength of light, effect, on dispersion, 

 5 

 on focus of lenses, 6 

 on photographic plate, 42 

 on resolution, 9, 10 

 Wess ( see Gray and Wess's medium ) 

 Wholemounts, diagram of technique for, 

 61,62 

 in gum media, 109, 110 

 of invertebrates, 111-113 

 of liver fluke, 189-192 

 of mite, 175 

 of Pectinatella, 178-182 

 in resinous media, 110-118 

 temporary, 108 

 of thick objects, 110 

 of 33-hour chick, 183-188 

 Working distance, 13 

 Wright's stain, 94 



applied to blood smear, 196-198 



Xylem, 96 



Xylene for clearing, 101, 129 



Ziehl-Neelsen technique, 204 

 Ziehl's carbolmagenta, 89 



applied to bacteria, 205 

 Zimmerman's lacquer, 167 

 Zenker's fixative, 71 



applied to rabbit liver, 239 



