CONTENTS 



Foreword vu 



Preface • xi 



Abbreviations xxiv 



Microscopic Techniques 1 



I. Freezing-Drying Preparation of Tissue 3 



Parker-Scott Method for Freezing-Drying Tissues 5 



II. Cliemical Methods U 



A. Requirements 11 



B. Inorganic Elements and Radicals 14 



Potassium 14 



Gersh Modification of Macallum Method for Potassium 14 



Carere-Comes Siena Orange Method for Potassium 15 



Calcium 16 



Calcium Sulfate Test for Calcium in Plant Tissue 17 



Cretin Color Test for Calcium and Other Minerals 17 



Von Kossa Silver Test for Calcium 17 



Magnesium ■ 18 



Broda Method for Magnesium 18 



Zinc 18 



Mendel and Bradley Method for Zinc 19 



Iron 19 



Prussian-Blue Test for Ferric Iron and TurnbuU's Blue for 



Ferrous Iron 20 



Humphrey Dinitrosoresorcinol Test for Iron 21 



Thomas and Lavollay Hydroxyquinoline Test for Iron 21 



Nickel 22 



Cretin and Pouyanne Method for Nickel 22 



Lead and Copper 22 



Mallorj' and Parker Hematoxylin Method for Lead and Copper 23 

 •• Mallory and Parker Methylene Blue Method for Lead and 



Copper 24 



Mercury 24 



Method of Hand et al. for Mercurous and Mercuric Mercury . . 25 



Silver 25 



Okamoto et al. Procedure for Silver 26 



Gold 27 



Roberts Method for Gold 27 



Elftman and Elftman Method for Gold 28 



Okamoto et al. Method for Gold ; 28 



Platinum 29 



Palladium 29 



Uranium 29 



Arsenic 30 



Castle Cupric Salt Method for Arsenic 30 



Bismuth 30 



Wachstein and Zak Method for Bismuth 31 



Castel Method for Bismuth 32 



XV 



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