Xvi CONTENTS 



Microscopic Techniques (continued) 



Chloride and Phosphate-Carbonate 32 



Gersh Method for Chloride and Phosphate-Carbonate 33 



Iodide 34 



Phosphate 34 



Serra and Queiroz Lopes Modification of the Molybdate 



Method for Phosphate 34 



Nitrate 35 



Cramer Method for Nitrate 35 



Sulfhydryl and Disulfide Groups 36 



Nitroprusside Test of Rapkine 36 



Bourne Nitroprusside Test 37 



Hammett and Chapman Nitroprusside Test 37 



Organic Substances and Groups 38 



Lipids and Cholesterol 38 



Kay and Whitehead Procedure for the Sudan IV Stain 

 for Lipids 39 



Jackson Procedure for Lipids Using Acetic-Carbol-Sudan III . . 39 



Telford Govan Technique for Sudan Dye Staining in 

 Aqueous Media 40 



Schultz Cholesterol Test 41 



Carotene, Carotenoids, and Vitamin A 42 



Steiger Method for Carotene in Leaves 42 



Riboflavin 43 



Chevremont and Comhaire Method for Riboflavin 43 



Polysaccharides in General 43 



Method of Hotchkiss for Polysaccharides 44 



Acid Polysaccharides— Hyaluronic Acid 45 



Hale's Method for Acid Polysaccharides 45 



Mucoproteins 46 



Lison's Method for Polysaccharide Sulfate Compounds (after 



Sylven) 47 



Glycogen and Mucin 47 



Bauer-Feulgen Stain for Glycogen (after Bensley) 48 



Best Carmine Stain for Glycogen (after Bensley) 49 



Gomori Procedure for Glycogen and Mucin 50 



Starch 51 



Milovidov Method for Starch 51 



Cellulose 52 



Post and Laudermilk (1942) Iodine Stain for Cellulose 52 



Chitin 52 



Murray Method for Softening Chitin 53 



Diaphanol Method for Softening Chitin 53 



Methods for Staining Chitin 54 



Ascorbic Acid 54 



Bourne Silver Stain for Ascorbic Acid 55 



Protein Reactions 56 



Arginine and Arginine-Containing Proteins 56 



Serra Method for Arginine and Arginine-Containing Proteins. . 57 



Thomas Method for Arginine and Arginine-Containing Proteins 58 

 Tryptophane in Proteins 58 



Romieu Reaction for Tryptophane in Proteins 59 



Tyrosine in Proteins 59 



Millon Reaction for Tyrosine in Proteins (after Bensley 



and Gersh) 59 



a-Amino Acid Groups in Proteins 60 



Berg Ninhydrin Test for o-Amino Acid Groups 60 



