CONTENTS XVll 



Microscopic Techniques (continued) 



Melanin 61 



Dublin Application of the Bodian Method to Demonstration 



of Melanin 61 



Hemoglobin 62 



Ralph Method for Hemoglobin 62 



GouUiart Method for Hemoglobin 62 



Dunn Method for Hemoglobin 63 



Bile Pigments and Acids 63 



Stein Test for Bile Pigments 63 



Forsgren Test for Bile Acids 64 



Aldehydes, Nucleic Acids, and "Plasmal" 65 



Coleman Preparation of Feulgen Reagent 67 



Whitaker Feulgen Technique for Plant Tissues 67 



Cowdry Modification of Feulgen Reaction for Paraffin 



Sections of Animal Tissues 68 



Oster Modification of Feulgen Reaction for Fresh-Frozen 



Sections of Animal Tissues 68 



Method of Turchini et al. for Nucleic Acids 69 



Water-Insoluble Carbonyl Compounds 69 



Bennett Use of Phenylhydrazine Reaction for Water-Insolu- 

 ble Aldehydes and Ketones 70 



Albert and Leblond Use of 2,4-Dinitrophenylhydrazine Re- 

 action for Water-Insoluble Aldehydes and Ketones 71 



Bennett Use of Semicarbazide Reaction for Water-Insoluble 



Aldehydes and Ketones 71 



Purines - 72 



Murexide Test for Certain Purines 72 



Hollande Modification of the Courmont-Andre Method for 



Uric Acid and Urates 73 



Indole and Related Compounds 73 



Phenols 74 



Lison Modification of the Chromaffin Reaction 74 



Urea 75 



Sulfonamides 75 



Method of MacKee et al. for Sulfonamides 76 



D. Enzymes 77 



Urease 77 



Alkaline Phosphatase 78 



Gomori Revised Method for Alkaline Phosphatase 79 



Acid Phosphatase 80 



Gomori Revised Method for Acid Phosphatase in Animal 



Tissues 81 



Glick and Fischer Adaptation to Grains and Sprouts of 



Gomori Method for Acid Phosphatase 82 



Other Phosphatases 85 



Zymohexase (Aldolase plus Isomerase) 86 



Allen and Bourne Method for Zymohexase 87 



Lipase 88 



Gomori Revised Method for Lipase 89 



Peroxidase 90 



McJunkin Method for Peroxidase in Tissue Sections 90 



Armitage Method for Peroxidase in Blood or Bone Marrow 



Smears 91 



Dopa Oxidase 91 



Laidlaw Method for Dopa Oxidase 92 



Amine Oxidase 93 



Oster and Schlossman Method for Amine Oxidase 93 



