CONTENTS XXI 



Chemical Techniques (continued) 



Amylase 302 



Proteolytic Enzymes 302 



Linderstr0m-Lang and Holter Acidimetric Acetone Method 

 for Proteolytic Enzymes 303 



Linderstr0m-Lang and Duspiva Alkalimetric Alcohol 

 Method for Proteolytic Enzymes 304 



Weil Formol Method for Tryptic Activity 306 



Arginase 306 



LinderstrcSm-Lang, Weil, and Holter Methods for Arginase. .307 

 Esterases and Lipases 308 



Glick Acidimetric Method for Esterase and Lipase 309 



Glick Acidimetric Method for Cholinesterase 310 



Catalase 310 



Holter and Doyle Method for Catalase 310 



IV. Gasometric Techniques 313 



A. Volumetric 313 



1. Capillary Respirometry 314 



(a) Cunningham-Barth-Kirk Differential Respirometer 314 



(b) Cunningham-Kirk Open-Tube Respirometer 319 



(c) Tobias-Gerard Respirometer 322 



(d) Scholander Micrometer-Burette Differential Respirometers 324 



2. Gas Analysis 326 



(a) Scholander Micrometer Burette Gas Analyzer 326 



(b) Berg Simplified Gas Analyzer 328 



(c) Scholander-Roughton Syringe Gas Analyzer 329 



Oxygen 331 



Roughton and Scholander Method for Oxygen 331 



Carbon Monoxide 334 



Scholander and Roughton Method for Carbon 



Monoxide 334 



Nitrogen 335 



Edwards, Scholander, and Roughton Method for 



Nitrogen 336 



Carbon Dioxide 337 



Scholander and Roughton Method for Carbon Dioxide. .337 



3. Membrane Interferometer Volumetry 340 



B. Manometric 342 



1. Cartesian Diver Manometry 342 



(a) Microliter Diver Technique 343 



(b) 0.1 Microliter or Capillary Diver Technique 382 



(c) Methods Other Than for Respiration 393 



Cholinesterase 393 



Lindestr0m-Lang and Glick Method for Cholinesterase . 393 



Thiamine and Cocarboxylase 394 



Westenbrink Method for Thiamine and Cocarboxylase 395 



Diphosphopyridine Nucleotide 396 



Anfinsen Method for Diphosphopyridine Nucleotide ...396 



2. Optical-Lever Respirometry 399 



C. Polarographic 404 



Microelectrode Measurement of Local Oxygen Tension in Tissue 404 



V. Dilatometric Techniques 413 



Dilatometric Apparatus and Its Use 414 



Peptidase 417 



Method of Linderstr0m-Lang and Lanz for Peptidase 419 



Density and "Reduced Weight" 420 



