XXll - CONTENTS 



Chemical Techniques (continued) 



VI. Determination of Amount of a Biological Sample 423 



A. Preparation of Frozen Tissue Sections of Accurate Thickness 427 



Linderstr0m-Lang and Mogensen Method for Accurate Cutting 

 and Special Handling of Frozen Tissue Sections 428 



B. Microscopic Examination and Chemical Analysis of the Same 



Tissue Section 430 



Method of Anfinsen et al. for Microscopy and Analysis on 

 the Same Tissue Section 431 



C. Volume of Irregularly Shaped, Small Biological Samples 431 



Holter Method for Measurement of Volume 432 



VII. Deductive Methods 435 



Microbiological Techniques 43 



Introduction 439 



Riboflavin 439 



Lowry and Bessey Method for Riboflavin 440 



Mechanical Separation of Cellular Components 445 



Introduction 447 



I. Types of High-Speed Centrifuges 448 



II. Separation of Components of A. punctulata Eggs (after Harvey) 452 



III. Isolation of Cell Nuclei 454 



A. Nuclei from Avian Erythrocytes 454 



Laskowski Procedure for Isolation of Nuclei 454 



Dounce and Lan Procedure for Isolation of Nuclei 455 



B. Nuclei from Other Cells 456 



Stoneburg Procedure for Isolation of Nuclei 456 



Dounce Procedure for Isolation of Nuclei 456 



Lazarow Procedure for Isolation of Liver Nuclei as Used by 



Hoerr 458 



Behrens Procedure for Isolation of Nuclei from Thymus and 



Lymph Cells (as Modified by Gulick et al.) 459 



IV. Isolation of Chromatin Threads from Cell Nuclei 461 



Claude and Potter Procedure . .v 461 



V. Isolation of Cytoplasmic Particulates 462 



A. Mitochondria 462 



Bensley and Hoerr Procedure for Guinea Pig Liver 462 



Claude Procedure for Certain Neoplastic Cells of the Rat 463 



Claude Procedure for Isolation of "Large Granules" from Liver 464 



B. Submicroscopic Particulates 465 



Lazarow Procedure for Separation and Isolation of Lipoprotein 



and Glycogen Particles from Guinea Pig Liver 466 



Claude Procedure for Isolation of "Microsomes" 466 



VI. Isolation of Chloroplasts from Leaf Cells 468 



Granick Procedure 469 



Neish Procedure 469 



VII. Isolation of Other Particulates from Cells 471 



Bibliography 473 



Bibliography Appendix 504 



List of Manufacturers 508 



Index 511 



