SUCCINIC DEHYDROGENASE 97 



10% sodium succinate and make up to 10 ml. with A//15 phos- 

 phate buffer, pH 7.6 to 8.0. 

 Control Medium. Omit the succinate in the substrate medium. 



PROCEDURE 



1. Prepare fresh frozen sections. 



2. Treat sections 10-15 min. with the substrate medium under 

 a cover slip, taking care to avoid air bubbles. Seal edges of cover 

 slip with paraffin to exclude air. 



3. Observe under microscope and compare with section in control 

 medium treated in the same fashion. 



Result. Fading of dye characterizes the enzyme activity. 



