MICROLITER DIVER TECHNIQUE 



381 



(at 24° z= 0.91; also valid with sufficient approximation at 22.5°); the other 

 designations as before. 



The chief practical significance of this equation is that it permits of the 

 determination of respiratory quotients (calculation of A^cOa when V q^ is 

 known from a parallel determination according to case 1). Since the deter- 

 mination of R.Q. calls for the highest possible accuracy, the correction term 

 for the solubility of CO2 in paraffin oil (T^^jj Z^'cOz) i^ ^^^ disregarded in the 

 above equation (as in case 2), though here too Vg^l is small in relation to V. 



The above formulas should be adequate for the calculation of most of the 

 experimental combinations occurring in practice. If in special cases some of the 

 experimental conditions to which the formulas correspond cannot be realized, 

 or if it is desired to use other gas combinations than those mentioned, then 

 the calculations of Linderstr0m-Lang (1943) will enable one to derive the 

 corresponding equations. 



Sample Calculation. In an experiment on the measurement of the respira- 

 tion rate of the amoeba Chaos chaos Linne, Holter (1943, page 467) obtained 

 the following data: 



Bottom drop, 0.4 /A. (Pringsheim soln. plus amoeba). 

 Lower neck seal, 0.4 fxl. (0.1 A'^ sodium hydroxide). 

 Paraffin oil seal, 0.4 fA. 



The curves in Figure 133 were plotted by Holter to show the time course of 

 the oxygen consumption, and from the data he made the following calculations : 



From the graphs: 



Diver 16: 



Diver 16: ^p (1 hr.) = 2.43 cm. 

 Diver 17: Ap (1 hr.) = 1.83 cm. 

 Diver 16a (control diver) = constant. 



18.07 + 0.4-0.87 + 0.8-1.0 - 0.4-1.325 - 0.8-1.325 - 



V = 



18.07-1.325 

 2.40 



O2 consumption = AF = 



1.325 

 5.77-2.43 273 



1000 273 + 22.5 



= 5.77m1. 

 12.9- 10-3 Ml-/hr. 



Diver 17: 



V = 16.09 + 0.4-0.87 + 0.8 -1.0 - 0.4-1.325 - 0.8-1.325 - 



16.09-1.325 

 2.40 



0-2 consumption = AF 



1.325 



5.11-1.83 



273 



1000 273 + 22.5 



= 5.11 m1. 

 8.6-10-3 juh/hr. 



