470 MECHANICAL SEPARATION OF CELLULAR COMPONENTS 



centrifugation. To obtain intact chloroplasts, substitute 0.5 M glu- 

 cose soln. for the distilled water in the procedure and centrifuge the 

 chloroplasts out at high speed without using the flocculating agent. 

 (Although nothing is said about it in Neish's paper, the general 

 experience with chloroplast isolation would suggest that the separa- 

 tions be carried out in the cold.) 



