x list of contents 



8. Smeae Preparations from Cut Surfaces 74 



Preparation of smears — typical preparation : Diagnostic smear of Negri 

 bodies. 



9. Squash Preparations from Solid Bodies 76 



The process of maceration — staining and mounting squash prepara- 

 tions — typical preparations: Macrosporocytes of Crocus, Dissociated Hydra. 



10. Ground Sections 80 



Preparation of the crude section — grinding and polishing agents — 

 typical preparations: Transverse section of bone, Section of coral with polyp 

 in situ. 



11. Sections of Free Material 88 



Nature of sections — microtomes for free sections — methods of holding 

 material — hardening and fixing material — staining and mounting sec- 

 tions — typical preparations: Transverse section of leaf of Ligustrum, Section 

 of wood. 



12. Paraffin Sections 94 



Selection of a fixative — dehydrating agents — clearing agents — embed- 

 ding media — technique of dehydrating, clearing and embedding — micro- 

 tomes — knives and knife sharpening — block mounting — cutting paraffin 

 ribbons — staining and mounting sections — cleaning and labelling slides — 

 typical preparations: Transverse section of frog's intestine, Section of 

 amphibian embryo. Sagittal section of whole mouse. 



13. Nitrocellulose Sections 142 



Nitrocellulose — preparation of nitrocellulose solutions — infiltration — 

 casting celloidin blocks — cutting sections — staining and mounting — 

 typical preparation : Transverse section of lily bud. 



14. Sections from Double Embedded Material 153 



Explanation of process — typical preparation : Sections, intended for recon- 

 struction, of pluteus larva. 



15. Frozen Sections 157 



Choice of a supporting medium — refrigerants — cutting frozen sections — 

 staining and mounting — typical preparation: Section of fatty tissue. 



16. Injections 162 



Selection of injection mass — methods of injection — typical preparations: 

 India ink injection of chicken embryo. Lead chromate injection of kidney glom- 

 eruli, Carmine-gelatin injection of intestinal capillaries. 



PART II— METHODS AND FORMULAS USED IN MAKING 



MICROSCOPE SLIDES 



Foreword to Part II 173 



