LIST OF CONTENTS ■ XI 



17. Preservatives (Referenced AS P) 175 



P 00 General observations — P 10 Preservatives miscible with water 

 — 11 inorganic reagents, 12 organic reagents, 13 other preservatives — P 20 

 Preservatives not miscible with water. 



18. Fixatives (Referenced as F) 182 



General observations — Standard fixative solutions — Formulas 

 arranged by classes — ^1 osmic, 2 platinic, 3 mercuric, 4 cupric, 5 picric, 6 

 chromic, 7 dichromate, S other inorganic salts, 9 other organic reagents: 

 alone, combined together, or with 0.1 formaldehj-de, 0.2 acetaldehyde, 0.3 

 acetone, 0.4 other mod'i&er : with or icithout 0.001 acetic, 0.002 trichloracetic, 

 0.003 formic, 0.004 nitric, 0.005 sulphuric, 0.006 hydrochloric, 0.007 oxahc, 

 0.008 other inorganic acids, 0.009 other organic acids — Basal fixative 

 solutions — Formulas arranged alphabetically. 



19. Accessory Fixative Formulas (Referenced as AF) 254 



AF 00 General observations — AF 10 fixative removers — AF 20 De- 

 calcifying AGENTS and AGENTS FOR SOFTENING CHITIN AF 30 BLEACHING 



AGENTS — AF 40 Macerating agents — AF 50 Narcotizing agents. 



20. Formulas and Techniques for Dye Stains of General Applica- 



tions (Referenced as DS) 267 



DS 00 General observations — DS 10 Dye staining techniques of 

 GENERAL APPLICATION — DS 11 NucLEAR STAINS, 11.1 hematoxylin (typical 

 preparations: Kat testis with iron-hematoxylin, Chicken embryo wholemount 

 with alum hematoxylin, Chicken embryo sections with acid-alum hematoxylin), 

 11.2 carmine (typical preparations: Liver fluke with carmalum, Medusa with 

 alcoholic borax-carmine, Chromosomes with iron aceto-carmine) , 11.3 other 

 natural dyes, 11.4 synthetic dyes (typical prepsirations: Pollen grains with 

 safranin, chromosomes with magenta) — DS 12 Plasma stains, 12.1 single con- 

 trast formulas, 12.2 double contrasts from one solution (typical prepara- 

 tions : Squalus embryo with 'picro-indigocarmine, Rat tongue with celestin blue — 

 picro-acid fuchsin) , 12.3 complex contrast formulas (typical preparations : 

 Section of earthworm with hematoxylin-acid fuchsin-anilin blue, Section of 

 mouse head with hematox^jlin- ponceau 2R-light green) — DS 13 Complex 



TECHNIQUES INVOLVING BOTH NUCLEAR AND PLASMA STAINING, 13.1 thiazin 



eosinates (typical preparation: Blood smear with methylene blue-azur A- 

 methylene violet-eosin Y), 13.2 thiazin eosinates with other dyes, 13.3 methyl 

 green techniques (typical preparation: Suprarenal body with methyl green- 

 acid fuchsin-orange G), 13.4 acid fuchsin techniques (typical preparation: 

 Section of Amphioxus with acid fuchsin-anilin blue-orange G), 13.5 safranin 

 techniques, 13.6 hematoxylin techniques, 13.7 other complex techniques. 



21. Formulas and Techniques for Dye Stains of Special Applica- 



tions (Referenced as DS) 374 



DS 20 Dye staining techniques op special application — DS 21 Sk- 

 LECTivE stains FOR HISTOLOGICAL ELEMENTS, 21.1 skeletal tissues (typical 

 preparations: Bones in wholemount of small salamander mth alizarin, Carti- 

 lage in embryo with methylene blue. Root skeleton with acid fuchsin-iodine 



