XU LIST OF CONTENTS 



green), 21,2 nervous tissues (t3T3ical preparations: Section of brain with 

 methylene blue, Section of spinal cord with hematoxylin. Neuroglia with crystal 

 violet), 21.3 blood, 21.4 other histological elements — DS 22 Stains foe 

 CYTOLOGiCAL ELEMENTS, 22.1 nuclei (typical preparation: Mitosis with rose 

 bengal-orange G-ioluidin blue), 22.2 mitochondria and Golgi (typical prepa- 

 ration: Mitochondria in pancreas with acid fuchsin-toluidin blue-aurantia) , 



22.3 Nissl granules, 22.4 yolk and fat granules, 22.5 plastids, 22.6 starch, 

 glycogen and amyloid granules, 22.7 mucin, 22.8 other cell inclusions and 

 extrusions — DS 23 Selective stains for specific organisms, 23.1 virus, 

 Rickettsiae and Negri bodies (typical preparations : Rickettsiae in guinea pig 

 scrotum with magentathionin, Negri bodies in guinea pig brain with ethyl 

 eosin-methylene blue), 23.2 bacteria (typical preparations: Bacterial smear 

 with crystal violet, Demonstration of Gram positive bacteria. Demonstration of 

 tubercle bacilli, Flagella of Proteus vulgaris, Diploccocci in liver of rabbit), 23.3 

 other parasites and commensals (typical preparations •.Pencillium mycelia in 

 orange rind with thionin-light green-orange G-erythrosin, Fungi in tissue 

 scrapings), 23.4 other animals, 23.5 other plants — DS 24 Miscellaneous 



TECHNIQUES. 



22. Accessory Dye Staining Formulas (Referenced as ADS) .... 514 



ADS 10 Mordants and tissue revivers, 11 miscellaneous formulas, 12 

 mordants — ADS 20 Differentiating solutions, 21 for hematoxylin, 22 

 for other stains. 



23. Formulas and Techniques for ]\Ietal Stains (Referenced as 



MS) 522 



MS 00 General observations — MS 10 Osmic acid, 11.0 typical prepa- 

 rations: (Golgi network in earthworm ovary), 11.1 staining solutions, 11.2 

 neurological techniques, 11.3 histological techniques, 11.4 techniques for 

 cell inclusions — MS 20 Gold — MS 21 Gold used alone, 21.0 typical 

 preparations: {Nerve termination in muscle), 21.1 staining solutions, 21.2 

 techniques — MS 22 Gold in combination with mercury, 22.0 typical 

 preparation (Protoplasmic neuroglia in the cerebral cortex), 22.1 staining 

 solutions, 22.2 neurological techniques — MS 23 Gold in other combina- 

 tions, 23.0 typical preparation (Spinal cord with ammonium dichromate — 

 gold chloride), 23.1 staining solutions, 23.2 neurological techniques, 23.3 

 cytological techniques, 23.4 other techniques — MS 30 Silver — MS 31 

 Silver nitrate, 31.0 typical preparations (Nervous elements of retina. 

 Neuroblasts and axons in chicken embryo, Spirochaetes in sections), 31.1 

 staining solutions, 31.2 neurological methods, 31.3 cytological methods, 



31.4 histological methods, 31.5 bacteriological methods — MS 32 Protein 

 silver, 32.0 typical preparation (Sciatic nerve of cat to show axis cylinders), 

 32.1 neurological methods, other methods — MS 33 Silver diammine, 33.0 

 typical preparations (Nerve endings in taste buds, Oligigodendria and mi- 

 croglia, microglia), 33.1 staining solutions, 33.2 neurological methods, 33.3 

 cytological methods, 33.4 histological methods, 33.5 bacteriological methods, 

 33.6 other silver diammine methods — MS 34 Silver in combination with 

 other metals, 34.0 typical preparations (Purkinje cells in the cerebellar 

 cortex. Structure of superior cervical ganglion. Neurons and dendrites in brain 



