176 



METHODS AND FORMULAS 



P 11.1-P 12.1 



as their composition. In the first group 

 (P 11.1) there are the few surviving 

 simple solutions of inorganic reagents. 

 These were once the most widely used 

 class of preservatives, but they are now 

 obsolete and scarcely ever employed, al- 

 though they are the easiest of all fluids to 

 seal. P 12.1 contains the organic reagents 

 as distinct from the inorganic and is 

 divided according to whether or not it 

 contains phenol (P 12.1 are the alcohols, 



aldehydes, glycerine, etc., without phenol; 

 12.2 contains the phenol mixtures). A 

 third group, P 12.3 below, contains 

 miscellaneous organic reagents including 

 complex mixtures of materials which, had 

 they been used singly, would have been 

 placed in groups P 12.1 or P 12.2. The 

 classification P 13.1 contains all the other 

 water-miscible preservatives which could 

 not justifiably have been placed in either 

 of the previous two classes. 



P 10 Formulas 



11 SOLUTIONS OF INORGANIC REAGENTS 



11.1 Formulas 



11.1 Assier test. 1882 Chevalier 



Chevalier 1882, 290 



formula: water 100, sodium chloride 10, acetic acid 1 



11.1 Boitard 1921a Boitard 1921, 49 



formula: water 100, ammonium alum 10, sodium chloride 11.5, mercuric chloride 1.2 



11.1 Boitard 1921b Boitard 1921, 379 



formula: water 86, sodium borate 8, boric acid 2, potassium nitrate 3, sodium chloride 1 



11.1 Goadby trst. 1855a Queckett Queckett 1855, 300 



formula: water 100, sodium chloride 6, potassium alum 3, mercuric chloride 0.15 



11.1 Goadby test. 1855b Queckett Queckett 1855, 301 



formula: water 100, mercuric chloride 0.08, ammonium alum 2.4, sodium chloride 4.8 

 note: Several other solutions were published by Goadby (Queckett, loc. cit.). These, 

 however, appear to be the only ones widely used (cf. Robin 1871, 377 and Frey 1877, 

 136) for microscopial, as distinct from anatomical, preservation. 



11.1 Kronecker test. 1907 Bohm and Oppel Bohm and Oppel 1907, 9 



formula: water 100, sodium chloride 0.6, sodium carbonate 0.006 



11.1 Ralf test. 1872 Martin Martin 1872, 189 



formula: water 100, potassium alum 0.2, sodium chloride 0.2 



11.1 Woods 1929 19938,70:637 



formula: water 100, copper acetate 0.5, acetic acid 4 

 method: Fix 4 hrs. in above. Pour off solution and add ammonia till color changes to 



purple. Return objects and leave 1 hr. Transfer to 5% glycerol, concentrate by 



evaporation, and mount in M 12.1 Kaiser 1880 

 RECOMMENDED FOR: algae. 



12 ORGANIC REAGENTS 



12.1 Alcohols, Aldehydes, Ketones 



23635, 30 :442 



12.1 Caberla 1878 



formula: water 30, glycerol 10, 95% ale. 20 



12.1 Francotte test. 1942 Langeron 



formula: water 50, 95% ale. 20, glycerol 30 



12.1 Gatenby and Painter 1937 



formula: water 50, glycerol 25, 95% ale. 25 



Langeron 1942, 1011 



Gatenby and Painter 1937, 222 



