F 0000.0010 FIXATIVES 189 



F 03 Formulas Arranged by Classes 



F 0000 SOLUTIONS WITHOUT PRIMARY OR 

 SECONDARY FIXATIVE AGENTS 



OOOO.OniO Acetic Alone 



Acetic acid is never used nowadays in simple aqueous dilution because it causes rapid 

 hydrolj'sis of the protoplasm. This hydrolysis is diminished by the presence of alcohol, there- 

 fore, the alcoholic dilutions given below can alone be recommended. These hydrolize cyto- 

 plasm rapidly, but do not greatly affect the nucleus. They are, therefore, mostly employed 

 for the demonstration of nuclei or of materials containing considerable nucleic acid. Acetic 

 acid is regarded by Gatenby and Cowdry (1928, 52) as "a substance most injurious to the 

 finer elements of the cytoi)lasni, but in some cases it is indicated for a study of nuclear ele- 

 ments." Langeron (1942, 73), on the contrary, regards it as one of the most important fixa- 

 tive agents. Unless a rapid hydrolysis of the cytoplasm is required to bring nuclear elements 

 into sharp contrast, it is strongly recommended that it be employed in mixture either with 

 chromic acid or with mercuric chloride. Spiess 1953 {in verb.) states that the substitution of 

 proprionic acid for acetic improves cytological fixation. 



0000.0010 Bartelmez 1915a 1135,25:87 



formula: abs. ale. 225, acetic acid 25 



0000.0010 Bartelmez 1915b 1135, 25:87 



formula: abs. ale. 237.5, acetic acid 12.5 



0000.0010 Bartelmez 1915c 1135, 25:87 



formula: ale. 152, chloroform 80, acetic acid 16 



0000.0010 van Beneden and Heyt 1877 3678, 14:218 



formula: abs. ale. 125, acetic acid 125 



0000.0010 Bradley 1948 20540b, 23 :29 



formula: abs. ale. 95, acetic acid 31, chloroform 125 



0000.0010 Carnoy 1887 6011, 3:6 



formula: abs. ale. 180, acetic acid 60 



0000.0010 Carnoy 1887 6011,3:276 



formula: abs. ale. 150, acetic acid 25, chloroform 75 

 note: Langeron 1934, p. 349 refers to this as "Carnoy or van Gehuchten." 



0000.0010 Farmer and Shove 1905a 17510, 48:559 



formula: abs. ale. 214, acetic acid 36 



0000.0010 Farmer and Shove 1905b 17510, 48:559 



formula: abs. ale. 167, acetic acid 73 



0000.0010 Favorsky 1930 766, 70: 376 



formula: 50%-80% ale. 235 to 250, acetic acid 15 to 1 

 note: Use strong acid /weak ale. for tough tissues and vice versa. 



0000.0010 Gilson 1897 



formula: abs. ale. 80, acetic acid 80, chloroform 80 



0000.0010 Hetherington 1922 11428,9:102 



formula: abs. ale. 100, chloroform 75, acetic acid 25, phenol q.s. to make 2.50 

 RECOMMENDED FOR: fixation and dehydration of nematodes. 



0000.0010 Lendrum 1935 11431,40:416 



FORMi la: water 10, abs. ale. 75, chloroform 10, phenol 4, acetic acid 3 



0000.0010 Moleschott 1871 see P 12.1 Moleschott 1871 



