196 METHODS AND FORMULAS F 1260.00I0-F 1300.0000 



1260.0010 Marchoux and Simond 1906 857, 20:105 



formula: water 250, osmic acid 1.5, platinic chloride 1.5, chromic acid 2, acetic acid 14 

 note: Poll 1910 (Ehrlich, Krause, et al. 1910, 2, 348) refers to this formula (without ref- 

 erence) as "Borrel's mixture." 



1 260.0030 Osmic-platinic-chromic-formic 



1260.0030 Pianese 1889 1886, 2 :412 



formula: water 250, osmic acid 1, platinic chloride 1.5, chromic acid 0.13, formic acid 

 0.5 



1268.0000 OSMIC-PLATINIC-CHROMIC- (other INORGANIC SALTs) 



1268.0000 Nebel 1934 7033a, 5:1 



REAGENTS REQUIRED: A. 0.1% ammonium hydroxide; B. 0.5% thorium nitrate; C. water 



230, osmic acid 5.75, platinic chloride 2.3, chromic acid 0.2 

 method: [root tips] -^ A, 5 mins. -^ 5, 3 hrs. -^ C, 24 hrs. 



1268.0020 Osmic-platinic-chro7nic-{other inorganic salt) -trichloroacetic acid 



1268.0020 Friedenthal 1908 test. circ. 1938 Wellings Wellings circ. 1938, 25 



formula: trichloroacetic acid 156, osmic acid 4, platinic chloride 4, chromic acid 8, 

 uranium acetate 78 



1270.0000 osmic-platinic-dichromate 



1270.0000 Veratti test. 1900 Golgi Golgi 1900, 2 :687 



formula: water 250, osmic acid 0.6, platinic chloride 0.08, potassium dichromate 4.0 



1270.0010 Osmic-'platinic-dichr ornate-acetic 



1270.0010 Johnson 1895 test. 1905 Lee Lee, 1905, 43 



formula: water 250, osmic acid 0.5, platinic chloride 0.4, potassium dichromate 4.3, 

 acetic acid 12.5 



1300.0000 OSMIC-MERCURIC 



These mixtures are cytological fixatives, and are worthless for nuclear preservation. They 

 are best applied to small invertebrates in which the rapid killing action of the osmic is 

 backed up by the hardening and mordanting action of the mercuric chloride. 



1300.0000 Apathy 1893 23632, 10 :349 



formula: water 250, osmic acid 2.5, mercuric chloride 9 



1300.0000 Apathy 1897 14246, 12 :495 



formula: water 250, osmic acid 1.25, mercuric chloride 8.75, sodium chloride 0.7 



1300.0000 Braun 1895 test. 1910 Poll Ehrlich, Krause, et al. 1910, 2 :351 



formula: water 250, osmic acid 0.013, mercuric chloride 17.5 



1300.0000 Biihler 1898 22302,31:316 



formula: water 250, mercuric chloride 17.5, osmic acid 0.125 



1300.0000 Heidenhain 1896 23632, 13:186 



formula: water 250, osmic acid 1.25, mercuric chloride 8.75 



1300.0000 Hirschler 1918 1780, 91 :140 



formula: water 250, osmic acid 2.5, mercuric chloride 8.75 



1300.0000 Kolster test. 1910 Poll Ehrlich, Krause, et al. 1910, 2:351 



formula: water 250, osmic acid 7.5, mercuric chloride 17.5, sodium chloride 1.25 



1300.0000 Mann 1894 23632, 11 :481 



formula: water 250, osmic acid 1.25, mercuric chloride 10, sodium chloride 2.25 

 note: The original method calls for equal parts of 1% osmic and a sat. sol. mercuric 

 chloride in 0.9% NaCl. 



