F 1300.0000-F 1370.0030 FIXATIVES 197 



1300.0000 Pappenheim 1896 1789a, 145 :587 



formula: water 250, osmic acid 2.5, niorcuric chloride 8.75 



1300.0010 Osmic-mercuric-acetic 



These are general-purpose fixatives, giving good preservation both of cytoplasmic and 

 nuclear elements, as well as being much easier for after-staining than are osmic-acetic mix- 

 tures alone. The mercuric chloride maj' assist in preserving the osmic acid against reduction. 



1300.0010 Colombo 1903 23632,20:282 



formula: water 250, osmic acid 1, mercuric chloride 7, acetic acid 0.5 



1300.0010 Cox 1896 23632, 13 :498 



formula: water 200, osmic acid, 1.0, mercuric chloride 7.0, acetic acid 50.0 



1300.0010 Druner 1894 10899,28:296 



formula: water 2-40, osmic acid 0.125, mercuric chloride 12.5, acetic acid 12.5 



1300.0010 Tschassownikow test. 1910 Spuler Ehrlich, Krause, et al. 1910, 2:526 



formula: water 245, osmic acid 1.25, mercuric chloride 13, acetic acid 6.25 



1300.0010 Zieglwallner 1911 test. 1928 Schmorl Schmorl 1928, 217 



formul.\: water 100, abs. ale. 125, osmic acid 1, mercuric chloride 3, acetic acid 25 



1340.0000 OSMIC-CUPRIC-MERCURIC 



1340.0000 Merton 1932 1798, 76:171 



STOCK, solutions: I. 2% copper sulfate; II. 2% osmic acid; III. sat. sol. mercuric chloride. 

 method: [Paramecium on smear of V 21.1 Mayer 1884] —> I, added to fluid on slide, 

 5 mins. -> drain -^ vapor of II, 1 min. -^ III, on slide 10 mins. — » 70% ale. 



1350.0010 OSMIC-MERCURIC-PICRIC-ACETIC 



1350.0010 Becher and Demoll 1913 Becher and Demoll 1913, 43 



formula: water 245, osmic acid 0.5, mercuric chloride 3.75, picric acid 0.75, acetic acid 5 



1350.0010 vom Rath 1895 766, 11 :285 



formula: water 250, osmic acid 0.5, mercuric chloride 8, picric acid 1.4, acetic acid 2.2 



1360.0010 OSMIC-MERCURIC-CHROMIC- ACETIC 



1360.0010 Brouha test. 1930 Guyer Guyer 1930, 150 



formula: water 240, osmic acid 5, mercuric chloride 11.5, chromic acid 5, acetic acid 

 12.5 



1360.0010 Cori lest. 1910 Spuler Ehrlich, Krause, ei al. 1910, 2 :526 



formula: water 250, osmic acid 0.003, mercuric chloride 15.3, chromic acid 0.08, acetic 



acid 0.03 

 note: This may also be prepared by mixing 7 parts of 7% mercuric chloride with 1 part 



of F 1600.0010 Cori 1890. 



1360.0010 Galescu 1908 6630, 65:429 



formula: water 250, osmic acid 0.04, mercuric chloride 4.4, chromic acid 0.5, acetic 

 acid 0.2 



1360.0010 Podwyssozki 1886 2526, 1 :287 



formula: water 250, osmic acid 0.5, mercuric chloride 1, chromic acid 2, acetic acid 5 



1370.0030 OSMIC-MERCURIC-DICHROMATE-FORMIC 



1370.0030 Charipper 1928 763, 38:401 



formula: water 240, osmic acid 1, mercuric chloride 10, potassium dichromate 5, 

 sodium sulfate 2, formic acid 10 



