F 1500.0050-F 1600.0000 FIXATIVES 199 



1500.0050 Francotte circa 1890 Francotte, 197 



formula: water 250, osmic acid 0.1, picric acid 0.5, sulfuric acid 5 



1500.0050 Schuberg 1903 23635, 74:155 



formula: water 250, osmic acid 0.4, picric acid 3, sulfuric acid 5 



1560.0000 OSMIC-PICRIC-CHEOMIC 



Attention must again be drawn to the improbability that picric acid will impart additional 

 qualities to an already good mixture, such as osmic-chromic alone. 



1560.0000 Fol 1896 test. 1910 Poll Ehrlich, Krause, et al. 1910, 2:348 



formula: water 250, osmic acid 0.01, picric acid 0.5, chromic acid 0.6 



1560.1010 Osmic-picric-chromic-acetic-formaldehyde 



1560.1010 Skovstedl933 1032,47:227 



formula: water 190, osmic acid 1, picric acid 1.5, chromic acid 2.8, acetic acid 9, 40% 

 formaldehyde 50, urea 3.8 



1580.0000 0SMIC-PICRIC-(0THER INORGANIC SALT) 



1580.0000 Perriraz 1905 5392, 61 :213 



formula: water 175, 95% ale. 75, osmic acid 0.12, picric acid 0.75, silver nitrate 0.75 

 preparation: Dissolve the osmic acid in the ale, and the salts separately in water. 

 Add heated (50°C.) picric solution to silver nitrate, then osmic. 



1600.0000 Osmic-chromic 



The osmic-chromic mixtures share with the following class the distinction of being the best 

 general-purpose fixatives. They give better cytological fixation than do the more strongly 

 acid osmic-chromic-acetic, but the considerable number of formulas included under each 

 class bear witness to their good qualities. When in doubt as to what fixative to choose for an 

 object with which one has had no previous experience, it cannot be too strongly recom- 

 mended that one from either this or the following class be employed. 



1600.0000 Barret 1886a 17510, 26:607 



formula: 50% ale. 250, osmic acid 0.5, chromic acid 0.5 



1600.0000 Barret 1886b 17510, 26:607 



formula: water 250, osmic acid 0.5, chromic acid 0.4 



1600.0000 Barret 1886c 17510, 26:607 



formula: water 250, osmic acid 0.25, chromic acid 0.65 



1600.0000 Bohm and Oppel 1896 see F 1600.0000 Flesch 1879 



1600.0000 Brock 1886 23635, 44 :333 



formula: water 250, osmic acid 0.04, chromic acid 0.25 



1600.0000 Drew 1920 see ADS 12.1 Drew 1920 



1600.0000 Flesch 1879 1780, 16 :300 



formula: water 250, osmic acid 0.25, chromic acid 0.6 



note: The formula given (without reference) by Bohm and Oppel in the 1896 edition of 

 their Taschenbuch does not differ significantly from this. 



1600.0000 Haug 1891 see AF 21.1 Haug 1891 



1600.0000 Lo Bianco 1890 14246, 9:443 



formula: water 250, osmic acid 0.05, chromic acid 2.5 



1600.0010 Osmic-chromic-acetic 



Flemming 1882 was the first of these excellent mixtures. The whole group is often referred 

 to as "Flemming." "Flemming without acetic" (FWA) is just that, and belongs in the last 

 class. 



