204 METHODS AND FORMULAS F 1700.0000-F 1700.1000 



1700.0000 Windle 1926 11135, 40:229 



formula: water 250, osmic acid 0.4, potassium dichromate 7.5 



1700.0000 Wlassow 1894 2526, 15 :543 



formula: water 250, osmic acid 0.1, potassium dichromate 0.6, sod. chloride 2.0 



1700.0000 Zietschmann 1903 23635,74:1 



formula: water 250, osmic acid 0.75, potassium dichromate 3.2, potassium sulfate 1.6 



1 700.001 Osmic-dichr ornate-acetic 



This is a much neglected group of general-purpose fixatives, indicated for histological study 

 in which it is desired to preserve the lipoid constituents of the cell. 



1700.0010 Bensley 1911 590, 12 :297 



formula: water 250, osmic acid 1, potassium dichromate 4, acetic acid 0.75 



1700.0010 Bensley and Bensley 1938 Bensley and Bensley 1938, 45 



formula: water 250, osmic acid 1, potassium dichromate 5, acetic acid 1.25 



1700.0010 Hoehl 1896 1739, (1896) :32 



formula: water 250, osmic acid 0.5, potassium dichromate 6, acetic acid 5 



1700.0010 Oxner 1905 10899, 40 :589 



formula: water 255, osmic acid 0.6, potassium dichromate 5.25, acetic acid 12.5 



1 700.0030 Osmic-dichromate-jorviic 



1700.0030 Oxner 1905 10899, 40 :589 



formula: water 255, osmic acid 0.6, potassium dichromate 5.25, formic acid 15 



1 700.0040 Osmic-dichr omate-nitric 



1700.0040 Dekhuyzen 1903 6628, 137:415 



formula: sea water 250, osmic acid 0.9, potassium dichromate 6.25, nitric acid 1.2 

 note: The original formula, which is said to be isotonic with sea water, requires 250 ml. 

 of a 2.5% solution of potassium dichromate in sea water, to be mixed with 25 ml. of 

 N nitric acid and 54 ml. of 2% osmic acid. 



1700.1000 Osmic-dichr omate-forvialdehrjde 



The apparently irrational mixtures that comprise this and the next few classes are in 

 reality of the utmost value, not only for the cytological and neurological studies for which 

 they were originally intended, but also for the fixation of embryos and of small invertebrates. 

 Where nuclear fixation is as important as cytoplasmic fixation, the acidified mixtures should 

 be used. 



1700.1000 Fish 1895 21400a, 17:319 



formula: water 250, osmic acid 0.025, potassium dichromate 2.5, 40% formaldehyde 

 0.5, potassium sulfate 2.5 



1700.1000 deirisola 1895 test. 1928 da Fano Gatenby and Cowdry 1928, 609 



formula: water 235, osmic acid 0.25, potassium dichromate 12.5, 40% formaldehyde 

 37.5 



1700.1000 Maximow 1909 23623,26:179 



formula: water 225, osmic acid 0.5, potassium dichromate 5, potassium sulfate 2.5, 

 40% formaldehyde 25 



1700.1000 Mislawsky 1913 23632, 81 :394 



formula: water 200, osmic acid 0.125, potassium dichromate 6, 40% formaldehyde 50 



1700.1000 Murray 1919 1200, 6:77 



formula: a. water 225, potassium dichromate 5.6, potassium sulfate 2.25, 40% formal- 

 dehyde 25; B. water 250, potassium dichromate 6.5, potasshim sulfate 2.5; C. water 

 250, osmic acid 5 



method: [whole organ or large piece] -^ A, overnight — + B, thin slices, 2-7 days -^ C, 2 

 days -^ running water, 12 hrs. 



