F 1700.1000-F 2000.1010 FIXATIVES 205 



1700.1000 Schridder (est. 1928 Gatenby and Cowdry Gatenby and Cowdry 1028, 330 



fcjumula: .1. water 225, potassium dichroiiiatc 5."), i)otassiuiii siilfato 2.25, 40% formal- 

 dehyde 25; B. water 250, potassium dichromate 0, potassiinu sulfate 2.5; ('. water 250, 

 osmic acid 5 



METHOD FOR MITOCHONDRIA AND FATS IN BIRDS AND M\MMAI,s: A, 2 days -* B, 2-4 days 



-^ (7, 2 days > running water, 24 hrs. 



1700.1000 Smirnow 1895 1780, 52:202 



formula: water 250, osmic acid 0.375, potassium dichroraate 9.5 



1 700.101 Osmic-dichromate-formaldehyde-ncetic 



1700.1010 Swank and Davenport 1934 20540b, 9:11 



formula: .1. distilled water 225, 40% formaldehyde 25; B. water 250, osmic aeitl 0.85, 

 potassium dichromate 0.34, potassium sulfate 0.8, acetic acid 5 



1780.0000 OSMIC-DICHROMATE-(oTHER INORGANIC SALTS) 



1780.0000 Cajal 1933a Cajal and de Castro 1933, 29 



formula: water 250, osmic acid 0.5, potassium dichromate 6, ferric chloride 12 



1780.0000 Cajal 1933b Cajal and de Castro 1933, 29 



formula: w^ater 240, osmic acid 0,4, potassium dichromate 4.8, potassium ferricyanide 

 1.2 



ISOO.OOOO 0SMIC-(0THER INORGANIC SALTs) 



1800.0000 Bensley and Bensley 1929 590, 44:79 



formula: water 250, osmic acid 5, ferric chloride 1.25 



1800.0000 Busch 1898 15058, 17:476 



formula: water 250, osmic acid 0.75, sodium iodate 2.5 



1800.0000 Kolossow 1898 1780, 52 :1 



formula: water 250, osmic acid 1.25, uranium nitrate 6.25 



1800.0010 (ind .0030 Osmic (icid-{othcr inorganic salts) -{acids) 



1800.0010 Frenkel 1893 766, 8 :539 



formula: water 250, osmic acid 1.25, acetic acid 1.25, palladium chloride 1.9 



1800.0030 Pianese 1899 1886, 2:412 



formula: water 250, osmic acid 1, cobalt chloride 2, formic acid 0.5 



F 2000 PLATINIC CHLORIDE IN COMBINATIOX WITH 

 FIXATIVE AGENTS OF HIGHER NUMERICAL RANK 



Platinic chloride is a valuable and neglected fixative agent. Its main value lies in its 

 mordanting power for after-staining, for it appears to interfere less with the staining proper- 

 ties of the tissues than any other reagent. It is stated by Langeron (1942, 372) to have all the 

 advantages of chromic acid wath none of the disadvantages resulting from the discoloration 

 of the tissue. It is particularly valuable in mixtures with chromic acid where it inhibits the 

 production of ehroniic oxide, which causes such gross discoloration and prevents good after- 

 staining. 



2000. ] 000 Platinic-formaldehyde 



2000.1000 Cajal 1893 test. Pollack 1900 Pollack 1900, 133 



formula: water 150, platinic chloride 0.08, 40% formaldehyde 100 



2000.1010 Platinic-formaldehyde-acetic 



2000.1010 Bouin 1900 1825, 17:211 



formula: water 200, platinic rhioride 1.8, 40% formaldehyde 50, acetic 12.5 



2000.1010 Retterer 1900 11024, 36:508 



formula: water 125, platinic chloride 6.25, 40% formaldehyde 125, acetic 7.5 



