F 2600.0000-F 3000.0000 FIXATIVES 207 



2600.0000 Platinic-chkomic 



The purpose of the platinic constituent of these mixtures is to prevent the discoloration of 

 tissues by the chromic acid. The fixative picture obtained does not differ from chromic alone, 

 or chromic-acetic, in the two classes here given. 



2600.0000 Eisig 1878 14246, 1 :341 



formula: water 250, platinic chloride 0.32, chromic acid 1.25 



note: This solution is frequently referred to Whitman who republished it (Whitman 

 1885, 153, 238) without reference. 



2600.0000 Merkel 1870 test. 1910 Poll Ehrlich, Krause, et al. 1910, 1 :224 



formula: water 250, chromic acid 0.5, platinic chloride 0.5 



2600.0000 Whitman 1885 see F 2600.0000 Eisig 1878 (note) 



2600.001 Platinic-chromic-acetic 



2600.0010 Brass 1884 23632, 1 :39 



formula: water 250, platinic chloride 0.35 to 0.85, chromic acid 0.35 to 0.85, acetic acid 

 0.35 to 0.85 



2600.0010 Lavdowsky 1894 764, 4:355 



formula: water 250, platinic chloride 0.12, chromic acid 2.4, acetic acid 12.5 



2700.0000 Platinic-dichromate 



2700.0000 Roncoroni test. 1900 Pollack Pollack 1900, 106 



REAGENTS REQUIRED: A. V 7000.0000 Midler 1859; B. 0.8% platinic chloride 



F 3000 MERCURIC CHLORIDE IN COMBINATION WITH 

 FIXATIVE AGENTS OF HIGHER NUMERICAL RANK 



Mercuric chloride is probably the best known and most widely used fixative agent. It has 

 many disadvantages. In the first place, it is a dangerous poison, which can be absorbed 

 through the skin (of those sensitive to it) and produce chronic, cumulative mercury poison- 

 ing. Unless the individual desiring to use these solutions is satisfied that he is not sensitive to 

 mercury, he should wear rubber gloves when handling the solution. Even those not sensitive 

 should take the utmost precautions to prevent contact of this dangerous material with the 

 bare skin. The second disadvantage is that once a material has been placed in mercuric solu- 

 tion, it cannot be handled with any metal instrument, but must be manipulated entirely 

 with instruments of glass, wood, or plastic. Mercuric chloride has also a tendency to render 

 materials brittle. The last disadvantage is that this reagent tends to cause a precipitate of 

 small crystals in the tissues unless it is washed out either by very prolonged washing in 

 water, or by relatively prolonged washing in solutions of iodine, which must then themselves 

 be removed by subsequent washing in alcohol. Against these disadvantages is the fact that 

 mercuric fixatives permit the most brilliant after-staining of almost any class. Mercuric 

 chloride is not usefully employed in simple aqueous solution, but is occasionally useful, in the 

 formulas which follow, in alcohol solution. 



3000.0000 Mercuric Alone 



3000.0000 Apathy test. 1920 Mayer Mayer 1920, 56 



formula: 50% ale. 250, mercuric chloride 9, sodium chloride 1.25 



3000.0000 Giemsa 1909 test. 1938 Mallory MaUory 1938, 41 



formula: water 165, 95% ale. 85, mercuric chloride 11.5 



3000.0000 Heidenhain 1888 16155, 43 (Suppl.): 40 



formula: water 250, mercuric chloride 22, sodium chloride 1.25 



3000.0000 Lenhossek 1899 test. circ. 1938 Welling Welling 1938, 29 



formula: water 115, 95% ale. 135, mercuric chloride 9, sodium chloride 11 



3000.0000 Lowit 1887 20170, 95:144 



formula: water 250, mercuric chloride 0.2, sodium sulfate 6, sodium chloride 2.5 



3000.0000 Neukirch 1909 see DS 22.6 Xeukirch lUOO 



