208 METHODS AND FORMULAS F 3000.0000-F 3000.0010 



3000.0000 Pietschmann 1905 1683, 16 :63 



formula: 90% ale. 250, mercuric chloride 0.9 



3000.0000 Prowazeko 1906 see F 3000.0000 Schaudinn 1893 (note) 



3000.0000 Rothig 1900 1780, 36 :354 



formula: water 225, 95% ale. 25, mercuric chloride 15.7 



3000.0000 Schaudinn 1893 13635, 57:19 



formula: 60% ale. 250, mercuric chloride 5.5 

 note: Prowazeko 1906 (23632, 23:1) specifies 90% ale. for the above solutions. 



3000.0000 Schaudinn 1900 23831, 13:197 



formula: 30 %> ale. 250, mercuric chloride 10 



3000.0000 Schmorl 1928 Schmorl 1928, 29 



formula: water 235, mercuric chloride 11, sodium chloride 1.25 



3000.0010 Mercuric-acetic 



The mercuric-acetic mixtures share with the chromic-acetic mixtures the honor of being the 

 most popular fixatives. It is probable that they deserve this. They are simple to make up, 

 stable in sohition, easy to handle, and do not harden as badly as does mercuric chloride alone. 

 They permit the most brilliant after-staining, and are widely used for the fixation of marine 

 forms. They can be confidently recommended to the inexperienced worker, provided that he 

 remembers to wash out the mercury thoroughlj' and not to handle the fixed object with any 

 metal instrument until washing is complete. 



3000.0010 Altman 1890 test. 1920 Mayer IMayer 1920, 59 



formula: water 200, mercuric chloride 3.5, acetic acid 50 



3000.0010 Apathy 1896 14246,12:495 



formula: water 150, ale. 100, mercuric chloride 5, acetic acid 1.25 



3000.0010 Beguin test. 1910 Spuler Ehrlich, Krause, et al. 1910, 521 



formula: water 225, mercuric chloride 16, acetic acid 25 



3000.0010 van Beneden test. 1905 Lee Lee 1905, 54 



formula: water 190, mercuric chloride to sat. (circ. 20), acetic acid 60 

 note: Both Lee 1905, 54 and Mayer 1920, 53 attribute this solution to van Beneden, but 

 neither quotes a reference. 



3000.0010 Bignami test. 1896 Kahlden and Laurent Kahlden and Laurent 1896, 127 

 formula: water 250, mercuric chloride 2.5, acetic acid 2.5, sodium chloride 2 



3000.0010 Borgert 1900 23831, 14:207 



formula: water 190, mercuric chloride 13.5, acetic acid 60 



3000.0010 Carazzi test. 1920 Mayer Mayer 1920, 56 



formula: 30% ale. 240, mercuric chloride 5.2, acetic acid 8.3 



3000.0010 Carnoy and Lebrun 1887 6011,13:68 



formula: abs. ale. 80, acetic acid 80, chloroform 80, mercuric chloride to sat. {circ. 60) 

 note: The original authors attribute this mixture to Gilson, by whom it was never pub- 

 lished. It is, however, frequently referred to as "Gilson's Mixture." Langeron 1934, 

 344 calls it "Gilson (1897)" but gives no reference. 



3000.0010 Carter 1919 1032,13:213 



formula: water 125, 95% ale. 125, mercuric chloride 7.5, acetic acid 7.5 



3000.0010 Cholodkowsky test. 1910 Spuler Ehrlich, Krause, et al. 1910, 2:521 



formula: water 250, mercuric chloride 17.5, acetic acid 1.25 



3000.0010 Coker 1902 3430, 33 :90 



formula: water 250, mercuric chloride 0.9, acetic acid 2.5 



