216 METHODS AND FORMULAS F 3670.0010-F 3700.0010 



3670.001 Mercuric-chromic-dichromate-acetic 

 3670.0010 Ruffini 1927 Ruffini 1927, 7 



formula: water 250, mercuric chloride 3, chromic acid 1.8, potassium dichromate 1.2, 



sodium sulfate 0.6, acetic acid 12 

 note: a second formula (loc. cit. p. 63) reduces the acetic acid to 3. 



3700.0000 Mercuric-dichromate 



Mercuric-dichromate fixatives are among the better known cytoplasmic reagents. They 

 are probably better for this purpose than the mercuric-chromics of the previous classes. 



3700.0000 Bensley 1910 763,4:379 



formula: water 125, 95% ale. 125, mercuric chloride 32, potassium dichromate 2.5 

 note: The original formula calls for a mixture of equal parts 2% dichromate and a sat. 



sol. IIgCl2 in abs. ale. Spuler 1910 (Ehrlich, Krause, ei al. 1.910, 2:254), quotes this 



formula as "Bensley 1896" but gives no further reference. 



3700.0000 Bensley and Bensley 1938 Bensley and Bensley 1938, 37 



formula: water 250, mercuric chloride 12.5, potassium dichromate 6.25 



3700.0000 Cox test. 1895 Rawitz Rawitz 1895, 17 



formula: water 250, mercuric chloride 2.5, potassium dichromate ■ 2.5, potassium 

 chromate 2 



3700.0000 Duthie 1937 see F 3700.0000 Lane (1910) (note) 



3700.0000 Foa 1891 test. 1920 Mayer Mayer 1920, 52 



formula: water 250, mercuric chloride 5, potassium dichromate 5, potassium sulfate 2.5 



3700.0000 Foa 1895 11360,37:287 



formula: water 250, mercuric chloride 10, potassium dichromate 6.25, sodium chloride 



1.25 

 note: Spuler 1910 (Ehrlich, Krause, et al. 1910, 2 :254) refers to this formula as "Nicki- 



forow-Foa" but gives no reference. 



3700.0000 Hoyer 1899 1780, 54 :97 



formula: water 240, mercuric chloride 4, potassium dichromate 3.2 



3700.0000 Lane test. 1910 Spuler Ehrlich, Krause, et al. 1910, 2:254 



formula: water 125, 96% ale. 125, mercuric chloride 32, potassium dichromate 4.4 

 note: The original calls for a mixture of equal parts 33^^% dichromate and a sat. 



sol. HgCl2 in 96%. Duthie 1937 (Gatenby and Cowdry 1937, 420) calls for 2,^% 



dichromate. 



3700.0000 Marrassini lest. 1910 Spuler Ehrlich, Krause, et al. 1910, 2:254 



formula: water 250, mercuric chloride 2.5, potassium dichromate 5.0 



3700.0000 Nickiforow see F 3700.0000 Foa 1895 (note) 



3700.0000 Salkind 1917 23632, 29 :540 



formula: water 250, mercuric chloride 10, potassium dichromate 6.25, chloral hydrate 

 10 



3700.0000 Wlassow test. 1910 Spuler Ehrlich, Krause, et al. 1910, 2:254 



formula: water 200, mercuric chloride 0.3, potassium dichromate 0.12, sodium chloride 

 6.0 



3700.0000 Wolbach 1919 11343,41:75 



formula: water 250, mercuric chloride 15, potassium dichi'omate 6.7 



3700.0010 Mer cur ic-dichr ornate-acetic 



These are some of the better general-purpose fixatives. The addition of acetic acid adds a 

 reasonable nuclear fixation to the excellent cytoplasmic fixation afforded by the principal 

 constituent. 



3700.0010 Bensley and Bensley 1938 Bensley and Bensley 1938, 44 



formula: water 237.5, mercuric chloride 12, potassium dichromate 6, acetic acid 12.5 



