F 3780. 1000-F 4000.1090 FIXATIVES 219 



S780.1000 Mercuric-dichromate-iother inorganic snUs)-forrnaldehyde 



3780.1000 Benoit 1922 G630, 86:1101 



formula: a. water 250, mercuric chloride 3.1, potassium dichromate 3.75, uranium 



nitrate 2.0, sodium chloride 0.5; B. 2% formaldehyde 

 method: [fix A, 4 hrs.] — > B, 12 hrs. — > wash 



8S00.1000 Mercuric-(other inorganic salts) 



3800.1000 Dominici 1905 8545, 2:219 



formula: water 220, mercuric chloride 15, 40% formaldehyde 30. Add sufficient tincture 

 of iodine to produce a deep wine color 



F 4000 CUPRIC SALTS IN COMBINATION WITH 

 FIXATIVE SALTS OF HIGHER NUMERICAL RANK 



Cupric salts are never used alone for fixation. They are good fixatives in general, owing 

 to their power of mordanting tissues, particularly those which it is desired subsequently to 

 stain in hematoxylin. 



4000.0010 CUPRIC-ACETIC 



4000.0010 Behrens 1898 test. 1920 Mayer Mayer 1920, 232 



formula: water 250, acetic acid 2.5, copper chloride 0.5, copper nitrate 0.5, phenol 2.5 



4000.0010 Ripart and Petit 1884 test. 1884 Carney Carnoy 1884, 94 



formula: water 125, camphor water 125, acetic acid 1.8, copper acetate 0.5, copper 



chloride 0.5 

 note: The camphor water employed is the pharmaceutical saturated solution of 



camphor in water; Mayer (Mayer, 1920, 232) suggests the substitution of thymol. 



4000.0020 Cupric-trichloroacetic 



4000.0020 Friedenthal 1907 20189, 209 



formula: water 190, copper acetate 8.75, trichloroacetic acid 60 



4000.0040 Cupric-nitric 



4000.0040 Petrunkewitsch 1933 19938, 77:117 



STOCK solutions: I. water 250, copper nitrate 30, nitric acid 20; II. water 250, phenol 



10, ether 15 

 WORKING solution: stock I 60, stock II 180 



4000.1000 Cupric-formaldehyde 



4000.1000 Gelderd 1909 6011,25:12 



formula: sea water 250, copper nitrate 5, 40% formaldehyde 12.5 



4000.1000 Stappers 1909 6011,25:356 



formula: water 220, copper nitrate 5, 40% formaldehyde 30 



4000.1010 Cupric-formaldehyde-acetic 



4000.1010 Emig 1941 Emig 1941, 71 



formula: methanol 75, water 67, copper acetate 0.75, 40% formaldehyde 75, acetic 



acid 7.5 

 recommended for: algae. 



4000.1090 Cupric-formaJdehyde-{other organic acids) 



4000.1090 Zirkle 1931 7033a, 2 :85 



formula: water 217.5, 40% formaldehyde 25, proprionic acid 7.5, copper hydroxide to 

 excess 



4500.1010 CUPRIC-PICRIC-FORMALDEHYDE-ACETIC 



The original purpose of Hollande in the mixture given below was to increase the picric 

 acid content of the well-known picric-formaldehyde-acetic of Bouin. The characteristics of 

 the fixative are, however, changed by the addition of copper, and it cannot be regarded as a 

 modified Bouin though it is found in some textbooks under this name. 



