222 METHODS AND FORMULAS F 5000.0010-F 5000.0050 



5000.0010 Deegener 1909 23831,27:634 



formula: 45% ale. 240, picric acid 1.6, acetic acid 12.5 



note: Deegener {loc. cit.) refers to this as "Zimmer's mixture," but quotes no reference. 

 Mayer 1920 indexes it as "Zimmer's Gemisch" but quotes the journal reference of 

 Deegener's paper. 



5000.0010 Dobell 1914 1798,34:133 



formula: 90% ale. 180, picric acid 40, acetic acid 67.5 



5000.0010 Eltringham fixative D—auct. Eltringham 1930, 46 



formula: 95% ale. 200, chloroform 37.5, picric acid 2, acetic acid 12.5 

 RECOMMENDED FOR: general insect histology. 



5000.0010 Gulick 1911 1820, 6:339 



formula: water 250, picric acid 1, acetic acid 7.5 



5000.0010 Hertwig lest. 1895 Rawitz Rawitz 1895, 22 



formula: water 100, picric acid 1.1 (to sat.), acetic acid 3-5 



5000.0010 Kingsbury and Johannsen 1927 Kingsbury and Johannsen 1927, 9 



formula: abs. ale. 200, chloroform 35, picric acid 2, acetic acid 15 



5000.0010 Potenza 1939 test. 1942 Langeron Langeron 1942, 419 



formula: water 125, picric acid 1.5, acetic acid 25, dioxane 100 



5000.0010 Zimmer 1909 see F 5000.0010 Deegener 1909 



5000 .0015 Picric-acetic-s u Ifu ric 



5000.0015 Blanc test. 1895 Maggi Maggi 1895, 112 



formula: water 100, picric acid 0.1, acetic acid 0.6, sulfuric acid 0.3 



5000.0015 Nemec 1899 10606,33:314 



formula: sat. sol. picric acid 250, acetic acid 1.25, sulfuric acid 1.25 



6000.0015 Patterson 1907 2975, 13 :252 



formula: F 5000.0050 Kleinenberg 1879 230, acetic acid 20 



5000.0020 Picric-trichloroacetic 



5000.0020 Cretin 1941 6630, 135 :355 



stock: I. dioxane 100, picric acid 25; II. water 35, trichloroacetic acid 5, methanol to 



make 100 

 WORKING solution: stock I 50, stock II 60 

 RECOMMENDED FOR: fixation of starch and glycogen in tissues held at 1-3°C. 



5000.0040 Picric-nitric 



5000.0040 Eltringham 1930 Eltringham 1930, 94 



formula: water 250, picric acid 2.6, nitric acid 10 



5000.0040 Kingsbury and Johannsen 1927 Kingsbury and Johannsen 1927, 9 



formula: water 237, nitric acid 13, picric acid q.s. to sat. 



5000.0040 Mayer 1880 14246,2:5 



formula: water 250, picric acid 4, nitric acid 6.25 



5000.0040 Zimmermann 1896 test. 1922 Schneider Schneider 1922, 323 



formula: a. 3% nitric acid; B. 1% picric acid in 95% ale. 



method: [plant material]—* A, 24 hrs. -^ water, 24 hrs. — * B, 12 hrs. -> 96% ale., 

 thorough wash 



5000.0050 Picric-sulfuric 



5000.0050 Kleinenberg 1879 17510, 19:208 



formula: water 250, picric acid 0.75, sulfuric acid 5 



note: Langenbeck 1898 (11373, 14:303) is this solution made with sea water. Mayer 

 1880 (14246, 2 :2) is the same solution as Kleinenberg, made another way; Mayer 1920 

 (p. 40) is incorrect in supposing it to be "stronger." 



