F 5000.0050-F 5000.1010 FIXATIVES 22-^ 



5000.0050 Langenbeck 1898 see F 5000.0050 Kleinenberg 1879 (note) 



5000.0050 Mayer 1880 see F 5000.0050 Kleinenberg 1879 (note) 



6000.0050 Wistinghausen 1891 1424G, 10 :47 



formula: water 250, picric acid 0.75, sulfuric acid 1.25 



5000.0060 Picric-hydrochloric 



5000.0060 Becher and Demoil 1913 Becher and Demoll 1913, 43 



formula: water 250, hydrochloric acid 20, picric acid q.s. to sat. 



6000.0060 Mayer 1880 1424G, 2 :5 



formula: water 250, picric acid 5, hydrochloric acid 5 



5000.1 000 Picric-formaldehyde 



These mixtures appear to have nothing whatever to recommend them but are still quoted 

 in the literature. 



6000.1000 Aniline 1903 test. 1920 Mayer Mayer 1920, 39 



formula: 70% ale. 225, picric acid to sat. (circ. 15), 40% formaldehyde 25 



6000.1000 Graf 1897 test. 1910 Blum Ehrlich, Krause, et al. 1910, 1 :489 



formula: water 225, picric acid 2.8, 40% formaldehyde 25 



note: Blum 1910 (Ehrlich, Krause, el al. 1910, 1:488) quotes four other "picric-formal- 

 dehydes of Graf"; this is the most usual. 



6000.1000 Mayer 1920 Mayer 1920, 31 



formula: sea water 200, picric acid 0.25, 40% formaldehyde 25 



5000.1000 Rossman test. 1947 Davenport cit. Deane, et at. 



20540b, 63, 401 

 formula: abs. ale. 225, picric acid 22.5, 40% formaldehyde 25 



5000.1000 Verhoeff 1926 test. 1938 Mallory Mallory 1938, 258 



formula: water 95, 95% ale. 130, picric acid 2.7, 40% formaldehyde 25 



5000.1010 Picric-formaldehyde-acetic 



These are today the most widely used general-purpose fixatives. They have nothing what- 

 ever to recommend them for any purpose save the demonstration of nuclei in meiosis, for 

 which the original Bouin 1897, undoubtedly the best-known fixative at present employed, 

 was developed. Bouin himself recommended his fluid for no other purpose than the demon- 

 stration of nuclear figures in the testis of the rat, and it is diflScult to understand how its use 

 has become so widespread. It is almost impossible to secure sharp cytoplasmic staining after 

 any of these mixtures. Their use should be confined entirely to that for which they were orig- 

 inally proposed, that is, the demonstration of nuclear figures. 



5000.1010 Allen 1929a Allen's PFAi—auct. McClung 1929, 425 



formula: water 190, picric acid 2.4, 40% formaldehyde 25, acetic acid 25 



5000.1010 Allen 1929b Allen's PFA<,—auct. MeClung 1929, 425 



formula: water 225, picric acid 2.8, 40% formaldehyde 12.5, acetic acid 12.5 



5000.1010 Allen 1929c Allen's PFA^—auct. McClung 1929, 425 



formula: water 190, picric acid 2.4, 40% formaldehyde 35, acetic acid 25, urea 2.5 



5000.1010 Bauer 1933 23639b, 33:143 



formula: dioxane 212.5, picric acid 75, 40% formaldehyde 25, acetic acid 12.5 

 RECOMMENDED FOR: preservation of glycogen in tissues. 



5000.1010 Becher and Demoll 1913 Becher and Demoll 1913, 48 



formula: water 25, 95% ale. 140, picric acid 2.6, 40% formaldehyde 67.5, acetic 

 acid 17.5 



