226 METHODS AND FORMULAS F 5000.1310-F 5600.1010 



5000.1310 van Walsen 1925 23632, 42 :439 



formula: water 130, picric acid 1.5, 40% formaldehyde 35, acetic acid 2.5, acetone 85 



5600.0000 Picric-chromic 



The addition of chromic acid to picric-acid mixtures probably undoes some of the damage 

 occasioned by the picric, in that the tissues after sectioning appear less vacuolated. The 

 presence of the picric acid, however, continues to cause diffused staining. 



5600.0000 Fol test. 1885 Whitman Whitman 1885, 237 



formula: water 250, picric acid 0.3, chromic acid 0.63 

 note: Gatenby and Cowdry 1928, 57 state that they have seen this formula "with the 



addition of a trace of acetic acid, quoted as 'liquid of Haensel'"; they do not say 



where or when. 



5600.0000 Haensel see F 5600.0000 Fol (1885) (note) 



5600.0000 Kahlden and Laurent 1896 Kahlden and Laurent 1896, 70 



formula: water 250, picric acid 0.25, chromic acid 0.375 



5600.001 Picric-chromic-acetic 

 5600.0010 Haensel see F 5600.0000 Fol (1885) (note) 



5600 . 00I^0 Picric-chro mic-nitric 



5600.0040 Rawitz 1895 Rawitz 1895, 24 



formula: water 250, picric acid 1, chromic acid 1.25, nitric acid 2 



5600.0050 Picric-chroviic-sulfuric 



5600.0050 Fol lest. 1910 Mayer Ehrlich, Krause, et al. 1910, 2:401 



formula: water 250, picric acid 0.5, chromic acid 0.88, sulfuric acid 3.3 



5600.0050 Lo Bianco 1890 14246, 9:443 



formula: water 250, picric acid 1.5, chromic acid 1.25, sulfuric acid 5 



5600.1 0000 Picric-chromic-formaldehyde 



5600.1000 Cohen 1935 20540b, 10:25 



formula: water 225, chromium sulfate 11.25, 40% formaldehyde 25, picric acid to sat. 

 note: Salicylic acid may be substituted for picric acid. 



5600.1000 Masson test. 1947 Paquin and Goddard cit. Warren 



4349, 27:195 

 formula: water 187.5, picric acid 2.2, chrome alum 7.5, 40% formaldehyde 67.5 

 preparation: Soak the alum in the formaldehyde for 1 hour before adding the picric 

 acid dissolved in the water. Leave for 24 hours and filter. 



5600.1010 Picric-chromic-formaldehyde-aceiic 



These are some of the best nuclear fixatives that have ever been developed, but they 

 should be confined exclusively to the fixation of tissues for the demonstration of nuclear 

 figures. They give very poor cytoplasmic fixation. 



5600.1010 Allen 1918 Allen's PF A,,— auct. 11373,31,135 



formula: water 190, picric acid 2.4, chromic acid 3.8, 40% formaldehyde 60, acetic 

 acid 7.5, urea 5 



5600.1010 Allen 1929a test. 1929 McClung Allen's Bx—auct. 



McClung 1929, 425 

 formula: water 190, picric acid 2.4, chromic acid 2.5, 40% formaldehyde 35, acetic acid 

 25, urea 2.5 



5600.1010 Allen 1929b lest. 1929 McClung Allen's PFAi^ or B,^—aucl. 



McClung 1929, 425 

 formula: water 190, picric acid 1.6, chromic acid 2.5, 40% formaldehyde 50, acetic acid 

 12.5, urea 2.5 



