228 METHODS AND FORMULAS F 6000.0010 



6000.0010 Chromic-acetic 



These are magnificent fixatives. The cytoplasmic fixation afforded by the chromic acid is 

 coupled with nuclear fixation by the acetic. The only disadvantage is that they tend to dis- 

 color the tissues, which sometimes renders after-staining difficult. They are as standard in 

 botanical practice as are the osmic-chromic-acetic mixtures for zoological purposes. 



6000.0010 Ammerman 1950 20540b, 25:197 



formula: water 238, chrome alum 3, 40% formaldehyde 30, acetic acid 2 

 RECOMMENDED FOR: heavily yolked embryos and insect larvae. 



6000.0010 Arnold 1888 1780, 31 :541 



formula: water 250, chromic acid 0.75, acetic acid 1.25 



6000.0010 Bataillon 1904 1756,18:1 



formula: water 225, chromic acid 2.25, acetic acid 25 



6000.0010 Becher and DemoU 1913 Becher and DemoU 1913, 24 



formula: water 100, chromic acid 0.25, acetic acid 0.1 



6000.0010 Claussen 1908 2626,26:144 



formula: water 250, chromic acid 1.25, acetic acid 2.5 



6000.0010 Czermak 1893 1823, 42 :581 



formula: water 250, chromic acid 0.625, acetic acid 2.5 



note: M'llroy and Hamilton 1901 (11024, 40) is identical. Yamanouchi 1906 (3430, 

 41 :425) requires the same ingredients dissolved in sea water. 



6000.0010 Demarbaix 1889 6011, 5:25 



formula: water 250, chromic acid 1.3, acetic acid 7.5 



6000.0010 Ehler test. 1910 Poll cit. Lee und Mayer Ehrlich, Krause, et al. 1910, 1:223 



formula: water 250, chromic acid 2, acetic acid 0.5 



note: Gatenby and Cowdry (1928, 36) quote, without reference, an "Ehler's Chrom- 

 acetic" which is identical with F 6000.0010 Lo Bianco 1890. 



6000.0010 Ehler see F 6000.0010 Lo Bianco 1890a (note) 



6000.0010 Faussek 1900 14246, 14:88 



formula: water 250, chromic acid 2.5, acetic acid 0.6 



6000.0010 Felix 1891 test. Poll 1910 Ehrlich, Krause, et al. 1910, 1:223 



formula: water 250, chromic acid 6.25, acetic acid 2.5 



6000.0010 Fick 1893 23635, 56 :529 



formula: water 250, chromic acid 1.25, acetic acid 0.25 



6000.0010 Flamming 1882 test. 1928 Gatenby and Cowdry 



Gatenby and Cowdry 1928, 35 

 formula: water 250, chromic acid 0.50, acetic acid 0.25 



6000.0010 Fol test. 1910 Poll Ehrlich, Krause, et al. 1910, 1:224 



formula: water 270, chromic acid 0.45, acetic acid 2.5 



6000.0010 Friedlander 1889 Friedlander 1889, 33 



formula: water 250, chromic acid 0.4, acetic acid 2.5 



6000.0010 Gates 1908 3430, 43 :81 



formula: water 250, chromic acid 1.75, acetic acid 1.25 



6000.0010 Gates and Latter 1927 11360, 47:280 



formula: water 250, chromic acid 2.5, acetic acid 2.5 

 note: Another formula in the same paper reduces the chromic acid to 1.5. 



6000.0010 Hertwig 1892 1780, 39 :353 



formula: water 250, chromic acid 2.5, acetic acid 0.5 



6000.0010 King test. 1910 Poll Ehrlich, Krause, et al. 1910, 1 :223 



formula: water 225, chromic acid 0.625, acetic acid 25 



