230 METHODS AND FORMULAS F 6000.0070-F 6000.1010 



6000.0070 Chromic-oxalic 



6000.0070 Graf 1898 test. 1910 Poll Ehrlich, Krause, el al. 1910, 1 :224 



formula: 30% ale. 250, chromic acid 0.75, oxalic acid 8 



6000.1000 Chromic-formaldehyde 



Chromic-formaldehyde mixtures must be prepared immediately before use, and it is 

 desirable to use them in the dark. Chromic-formaldehyde alone, without acidification, is of 

 no value save for cytoplasmic fixation. 



6000.1000 Braus 1896 7137, 6:307 



formula: water 180, chromic acid 0.625, 40% formaldehyde 60 



6000.1000 Levitsky 1910 2G26 28 :540 



formula: water 225, chromic acid 0.4, 40% formaldehyde 25 



6000.1000 Lo Bianco 1890 14246, 9:443 



formula: water 125, sea water 110, chromic acid 1.25, 40%, formaldehyde 12.5 



6000.1000 Marina 1897 19443, 2 :20 



formula: 90%, ale. 250, chromic acid 0.25, 40%, formaldehyde 12.5 



6000.1000 Nemec test. 1937 Gatenby and Painter Gatenby and Painter 1937, 678 



formula: water 232, chromic acid 2.3, 40% formaldehyde 18 



6000.1000 Prokofieva 1934 7033a, 5 :498 



formula: water 190, chromic acid 6.25, 40%, formaldehyde 60 



6000.1 01 Chromic-formaldehyde-acetic 

 This class of fixative is widely shown to botanists under the generic name GRAF. 



6000.1010 Belling 1928 20540b (abstr.) 4:94 



formula: water 185, chromic acid 2.5, acetic acid 25, 40% formaldehyde 40 



6000.1010 Belling 1930 BeUing 1930, 244 



formula: stock I. water 212, chromic acid 3.5, acetic acid 38; stock II. water 117, 



40% formaldehyde 133; stock III. water 185, 40% formaldehyde 65 

 working solutions: A. stock I 125, stock II 125; B. stock I 125, stock III 125 

 RECOMMENDED FOR: Usc solution A for general nuclear fixation; solution B for metaphase 



preparations only. 



6000.1010 Destin test. 1929 McClung and Allen McClung 1929, 422 



formula: water 250, chromic acid 2.5, 40% formaldehyde 15, acetic acid 5 



6000.1010 Gatenby and Painter 1937 see F 6000.1010 Navashin 1912 (note) 



6000.1010 Guyer 1930 Guyer 1930, 218 



formula: water 160, chromic acid 1.6, acetic acid 10, 40% formaldehyde 85 



6000.1010 Karpenchenko 1924 14,387:11211 



formula: water 235, chromic acid 1, acetic acid 7.5, 40% formaldehyde 7.5 

 note: This formula is attributed to "Navashin 1924" by Semmens 1939 {Microscope, 

 3:4). 



6000.1010 Marchoux 1910 test. 1928 Gatenby and Cowdry 



Gatenby and Cowdry 1928, 65 

 formula: water 120, chromic acid 0.8, 40% formaldehyde 120, acetic acid 75. 

 note: Gatenby and Cowdry (lac. cit.) cite this formula as "from Perez 1910 (1915, 

 5:11)." The journal reference is incorrect and the writer has never found any other 

 source for the formula. 



6000.1010 Navashin 1912 23253, 42, 28 



formula: water 188, chromic acid 2, 40% formaldehyde 50, acetic acid 12 

 note: Gatenby and Painter 1937, 677 recommend the reduction of the chromic acid 

 to 1.6. 



