19 



Accessory Fixative Formulas 



Decimal Divisions Used in Chapter 



AF 00 General observations and explanation of decimal divisions 

 AF 10 Fixative removers 



11 For use after picric mixtures 

 11.1 Formulas 



12 For use after mercuric mixtures 

 12.1 Formulas 



13 For use after other fixatives 

 13.1 Formulas 



AF 20 Decalcifying agents and methods for softening chitin 



21 Acid media for decalcification 

 21.1 Formulas 



22 Alkaline or neutral media for decalcification 

 22. 1 Formulas 



23 Methods for softening chitin 

 23.1 Formulas 



AF 30 Bleaching agents 



31.1 Formulas 

 AF 40 Macerating and digesting agents 



41 Methods involving use of hydrolysis 



41.1 Formulas for acid hydrolyzing solutions 



41.2 Formulas for alkaline hydrolyzing solutions 



41.3 Formulas for neutral hydrolyzing solutions 



42 Methods using enzyme digestion 

 42.1 Formulas 



AF 50 Narcotizing agents 

 51.1 Formulas 



AF 00 General Observations and Classification of Formulas 



The formulas included in this chapter solutions. The formulas are arranged ac- 



are those which are used either immedi- cording to the use for which they are in- 



ately before, or immediately after, fix- tended, and therefore fall logicall}' into 



ation, and which would therefore seem the classification indicated above, 

 to warrant the title Accessory fixative 



AF 10 Fixative Removers 



Most fixatives may be removed from nation of picric acid with various of the 



tissues by the simple process of washing proteins present. Some of these picric 



either in alcohol or in water. The principal compounds are soluble in water, and if 



exceptions to this statement are fixatives picricmaterialbe washed for a long period, 



containing picric acid or mercuric chloride, many of the cells will be found to be 



Much of the yellow picric stain cannot be heavily vacuolated. This may be required 



removed, since it results from the combi- if studies of the nucleus only are being 



254 



