AF 21.1 ACCESSORY FIXATIVE FORMULAS 257 



21.1 "Bielschowsky-Agduhr" see ADS 21.1 Cajal 1933a (note) 



21.1 Bodecker 1937 11147,16:143 



formula: methanol 100, celloidin 6, nitric acid 4.5 

 RECOMMENDED KOR: euaniol of teeth. 



21.1 Brain 1950 11300, 70:313 



formula: water 100, formic acid 5, calcium phosphate 2 

 RECOMMENDED FOR: teeth (sec E 24.1 Brain 1950, Chapter 27). 



21.1 Busch 1877a 1780,14:481 



formula: water 100, chromic acid 2, hydrochloric acid 3 



21.1 Busch 1877b 1780,14:481 



REAGENTS REQUIRED: A. watcr 100, chromic acid 0.1, potassium dichromate 1; B. water 



100, potassium dichromate 1, nitric acid 2 

 method: [fixed tissues] — > A, several days — > B, till decalcified — > rinse — > 95% ale. 



21.1 Cajal 1933a Cajal and de Castro 1933, 39 



reagents required: A. 20% formaldehyde; B. 4% nitric acid; C. 80% pyridine 

 method: [fresh tissue] —> A, 1-3 days -> B, till decalcified-^ wash, 24 hrs. running 



water -^ C, 24 hrs. — > 95% ale, till pyridine removed. 

 note: The method attributed, without reference, to "Bielschowsky-Agduhr" by Cajal 

 1933, 40 differs from the above only in the omission of C. 



21.1 Cajal 1933b Cajal and de Castro 1933, 40 



formula: water 86, 40% formaldehyde 11, nitric acid 3 



21.1 Carleton and Leach 1938 Carleton and Leach 1938, 211 



REAGENTS REQUIRED: A. Water 80, nitric acid 10, 40% formaldehyde 10; B. 5% sodium 



sulfate 

 method: [fresh or fixed bones] -^ A, till decalcified -+ B, till free from acid — > [sections] 



21.1 de Castro 1925a 21344,23:427 



formula: water 65, ale. 35, chloral hydrate 5, nitric acid 3 



21.1 de Castro 1925b 21344,23:427 



formula: water 40, 95% ale. 60, urethan 2, nitric acid 3 



21.1 de Castro 1925c 21344, 23:427 



formula: water 40, 95% ale. 60, aprobarbital 2, phenobarbital 2, nitric acid 4 

 note: Those solutions are primarily intended for materials subsequently to be metal 

 stained. Tlie author has translated the hypnotics and sedatives from European 

 proprietary trade names to the terms preferred in contemporary American usage. 



21.1 de Castro 1926 21344, 23:427 



formula: water 50, 95% ale. 50, chloral hydrate 2.5, nitric acid 3.4 



RECOMMENDED FOR: use before silver staining of nerves in bone or teeth. ^ 



note: 1 urethan or 0.6 diethylamine barbiturate may be substituted for chloral hydrate, 

 in which case the alcohol should be increased to 60%. 



21.1 Cretin 1925 Le Mans, 48 



formula: water 90, potassium iodide 22.5, iodine 25, trichloroacetic acid 10 

 recommended for: decalcification after F 3500.1000 Cretin 1925 fixation. 



21.1 David 1935 19938, 82:179 



reagents keqiured: A. 10%, nitric acid; B. 5% sodium sulfate; 

 method: [fresh bone] -^ A, till decalcified^ B, 24 hrs. — > running water, 24 hrs. 



21.1 von Ebner test. 1891a Haug 23632, 8:3 



formula: water 100, nitric acid 2, sodium chloride 18 

 note: Add 1 nitric acid daily until decalcification complete. 



: ( 



21.1 von Ebner test. 1891b Haug 23632, 8; 



formula: water 15, 95% ale. 85, hydrochloric acid 0.4, sodium chloride 0.4 



