AF41.1 ACCESSORY FIXATIVE FORMULAS 263 



41.1 Drost test. 1895 Rawitz Rawitz 1895, 7 



formula: sea water 100, osmic acid 0.05, chromic acid 0.125, acetic acid 0.05 



41.1 Felix test. 1942 Langeron cit. Mann Langcron 1942, 351 



formula: 30% ale. 100, salicylic acid to sat. 



41.1 Freude 1879 20170, 78:102 



formula: water 50, glycerol 35, hydrochloric acid 15 



41.1 Gage 1892 21400a, 14:120 



formula: water 75, 95% ale. 25, picric acid 1 



41.1 Goodrich 1942 17510,83:245 



formula: water 100, ADS 12.2 Lugol 0.25, sodium chloride 0.9, boric acid to sat. 



41.1 Haller 1887 14555, 11 :321 



formula: water 50, glycerol 25, acetic acid 25 



41.1 Harlow 1928 3420,85:226 



REAGENTS REQUIRED: A. sat. aq. sol. chlorine; B. 3% sodium sulfite 



method: [match-size pieces of wood] -^ boil -^ A, 2 hrs. —^ B, 80°-90°C., 15 mins. -^ 



[repeat A-^ B cycle till wood fails to turn red in B] —* wash —y section or tease 

 recommended for: separation of wood fibers. 



41.1 Hertwig 1879 see F 1000.0010 Hertwig 1879 (note) 



This is given as a macerating agent by Gatenby and Painter 1937, 246. It was intended 

 in the original as a fixative. 



41.1 Hertwig test. 1895 Rawitz Rawitz 1895, 7 



formula: water 100, osmic acid 0.025, acetic acid 1 



41.1 Hopkins test. 1895 Rawitz Rawitz 1895, 7 



reagents required: A. 20% nitric acid; B. sat. aq. sol. potassium alum 

 method: [fresh tissues] -^ A, till soft, — » wash -^ B, till sufficiently disintegrated 



41.1 Klebs test. 1895 Rawitz Rawitz 1895, 9 



formula: water 100, sucrose 5, sulfuric acid 5 



41.1 Konigstein 1895 20170 (1895) 71 



formula: water 30, glycerol 30, hydrochloric acid 30 



41.1 Kuhne 1862 test. 1895 Ranvier Ranvier 1895, 79 



note : Under this reference, also cited by Gatenby and Painter 1937, 246, a method of pro- 

 ducing a serious explosion is described. The original, which must surely be misquoted, 

 is not available to the author. 



41.1 Ludwig 1872 test. 1883 Cole Cole 1883, 135 



formula: 90% ale. 80, hydrochloric acid 20 

 method: [vertical slices of fresh kidney] -^ macerant, boil with reflux, 1-3 hrs. — > leave 



to settle -^ decant — > add water, leave 24 hrs. -^ shake — * mount selected tubules. 

 recommended for: isolation of renal tubules. 



41.1 MacCallum 1900 test. 1905 Bohm and Davidoff Bohm and Davidoff 1905, 23 

 formula: water 40, glycerol 40, nitric acid 20 



41.1 Masson 1929 4349, 12:81 



formula: 95% ale. 90, nitric acid 10 



41.1 Mobius 1887 see F 1600.0010 Mobius 1887 (note) 



41.1 Rogers test. 1927 Kingsbury and Johannsen Kingsbury and Johannsen 1927, 82 



formula: water 80, picric acitl I, acetic acid 20 

 recommended for: disassociation of striped muscle. 



