264 METHODS AND FORMULAS AF 41.2-42.1 



AF 41.2 Formulas for Alkaline Hydrolyzing Solutions 



41.2 Behrens, Kossel and Schiefferdecker 1889 Behrens, Kossel and Schieffer- 



decker 1889, 156 

 REAGENTS REQUIRED: A. water 67.5, potassium hydroxide 32.5; B. water 50, acetic acid 



50 

 method: [fresh tissue] — » A, on shde, till macerated — * drain -^ B, dropped on slide — > 

 DS 11.2 stain if required -^^ M 11 or M 12 mountant. 



41.2 Reinke 1892 766, 8:582 

 formula: water 50, glycerol 20, 95% ale. 20, lysol 10 



AF 41.3 Formulas as for Neutral Hydrolyzing Solutions 



41.3 Arnold 1898 1780, 52:135 

 formula: water 100, potassium iodide 10, iodine 0.15 



41.3 Gage 1895 test. 1896 ips. Gage 1896, 177 



formula: water 99.5, 40% formaldehyde 0.5, sodium chloride 0.6 



41.3 Gage 1897 21400a, 19:179 



formula: water 100, potassium dichromate 0.25, sodium sulfate 0.1, sodium chloride 0.9 



41.3 Landois 1885 tt^st. 1937 Gatenby and Painter Gatenby and Painter 1937, 245 



REAGENTS REQUIRED: .4. Water 100, ammonium chromate 1.25, potassium phosphate 



tribasic 0.5, sodium sulfate 0.6; B. solution A. 50, DS 11.26 Beale 1857 50 

 note: Gatenby and Painter give a reference to "1780, n.v., 445" which is incorrect. 



41.3 Moleshott and Borine 1872 test. 1875 Ranvier Ranvier 1875, 242 



formula: water 100, 95% ale. 20, sodium chloride 1 



41.3 Schiefferdecker 1886 1780, 28 :305 



formula: water 60, glycerol 30, methanol 3 

 note: This formula was republished by the same author in 1911 (23632, 28:318). 



41.3 Soulier 1891 test. 1905 Lee Lee 1905, 302 



formula: F 4000.0010 Ripart and Petit 50, 2% sodium thiocyanate 50 

 note: Soulier is reported (loc. cit.) to have added thiocyanates to a large number of 

 fixatives. The mixture here given is thought by Lee to be the best. 



AF 42 METHODS USING ENZYME DIGESTION 



AF 42.1 Formulas 



42.1 Faberge 1945 20540b, 20:1 



preparation: Extract stomach contents of Helix pomatia. Preserve with 1 drop toluene. 

 recommended for: removal of cell wall from plant tissues for smear preparation of 

 chromosomes. This technique is described in detail in Chapter 9. 



42.1 Hoehl 1897 1739, n.v., 136 



formula: water 100, sodium carbonate 0.3, pancreatin 0.25 



42.1 Jousset 1903 1863, 15 :289 



formula: water 100, glycerol 1, hj'drochloric acid 1, sodium fluoride 0.3, pepsin 0.1 



42.1 Kuskow 1895 1780,30:32 



formula: water 100, pepsin 0.5, oxalic acid 3 



42.1 Langeron 1942 Langeron 1942, 353 



formula: water 100, sodium hydroxide 0.2, pancreatin 0.3, thymol to sat. 

 note: Use at 37°C. 



