20 



Formulas and Techniques for Dye Stains 

 of General Application 



Decimal Divisions Used in Chapter 



DS 00 GENERALITIES 



01 General observations 



02 Method of arrangement of formulas 



DS 10 DECIMAL DIVISIONS USED FOR DYE STAINING TECHNIQUES OF 

 GENERAL APPLICATION 

 11 Nuclear staining techniques 



11.1 Hematoxylin stains 

 11.10 Tj^pical examples 



Demonstration of spermatogenesis in the rat testis using the 

 iron hematoxylin stain of Heidenhain 1892 

 Preparation of a wholemount of a 48-hour chicken embryo using 

 the alum hematoxylin stain of Carazzi 1911 

 Preparation of a series of demonstration slides, each having six 

 typical transverse sections of a 72-hour chicken embryo, using 

 the acid alum hematoxylin stain of Ehrlich 1896 

 Till Mordant hematoxylin staining 



11.111 After ferric alum mordants 



11.112 After ferric chloride mordants 



11.113 After other mordants 

 11.12 Direct hematoxylin staining 



11.121 Formulas incorporating iron mordants 



11.122 Formulas incorporating alum mordants 



11.123 Formulas incorporating acid alum mordants 



11.124 Formulas incorporating other mordants 



11.2 Carmine stains 



11.20 Typical examples 



Preparation of a wholemoimt of a liver fluke using the "car- 



malum" stain of Mayer 1897 



Preparation of a wholemount of a medusa using the alcoholic 



borax-carmine stain of Gronacher 1879 



Preparation of a smear to show chromosomes in salivary glands 



of Chironomus using the iron-aceto-carmine stain of Belling 1921 



11.21 Alum carmines 



11.22 Alcoholic carmines 



11.23 Acetocarmines 



11.24 Picro-carmines 



11.25 Iron carmines 



11.26 Ammonia carmines 



11.27 Hydrochloric^ carmines 



11.28 Other carmine formulas 



11.3 Brazilin and other natural stains 



267 



