268 METHODS AND FORMULAS 



11.4 Synthetic nuclear stains 



11.40 Typical examples 



Preparation of a strewn slide of pollen grains using the safranin 

 stain of Johannsen 1940 



Demonstration of chromosomes of the grasshopper in a smear 

 preparation of the testes, using magenta by the method of 

 Henneguy 1891 



11.41 Soluble metallic lakes of the oxazines, etc. 



11.42 Safranin 



11.43 Magenta 



11.431 Magenta as dye 



11.432 Magenta as leucobase 



11.44 Thiazines 



11.45 Crystal violet 



11.46 Other synthetic nuclear stains 

 12 Plasma staining techniques 



12.1 Single contrast formulas 



12.11 Aqueous solutions 



12.12 Weak alcohol solutions 



12.13 Strong alcohol solutions 



12.14 Clove-oil solutions 



12.15 Phenol solutions 



12.16 Other solutions and mixtures 



12.2 Double contrasts from one solution 



12.20 Typical examples 



Preparation of a transverse section of a Squahis embryo using 

 the picro-carmine stain of Rarivier 1899 followed by the picro- 

 indigo-carmine of Cajal 1905 



Preparation of the transverse section of the tongue of a rat 

 using coelestin blue followed by the picro-acid-fuchsin of van 

 Geisen, 1896 



12.21 Contrasts for red nuclei 



12.211 Formulas containing picric acid 



12.212 Other formulas 



12.22 Contrasts for blue nuclei 



12.221 Formulas containing picric acid 



12.222 Other formulas 



12.3 Complex contrast formulas 



12.30 Typical examples 



Preparation of a transverse section of an earthworm using the 

 iron hematoxylin stain of Regaud 1910 followed by the acid 

 fuchsin-aniline blue of Masson 1912 



Preparation of a transverse section of the head of a mouse using 

 an acid alum hematoxylin stain (Masson 1934) followed by 

 ponceau 2R-light green (Patay 1934) 



12.31 Techniques employing the phosphotungstic (molybdic) reaction 

 with fuchsin 



12.32 Techniques employing the phosphotungstic (molylxlic) reaction 

 with other dyes 



12.33 Other complex contrasts 



13 Complex techniques involving both nuclear and plasma staining 



13.1 T<'chiii(iuo8 employing the oosinates of the thiazins without other 

 admixture 



13.10 Typical example 



Preparation of a blood smear using the methylene blue-azur A- 

 methylene violet-eosin Y stain of Kingsley 1935 



13.11 Methylene blue eosinates 



13.12 Polychrome methylene blue eosinates 



13.13 Other thiazine eosinates 



