DS ll.II-DS 11,111 DYE STAINS OF GENERAL APPLICATION 281 



commonly employed is still that of Heidenhain 1892, from which the majority of the other 

 formulas have been derived. These variations are for the most part in the concentration 

 of the solutions employed or in the temperature at which they are used. The technique of 

 R^gaud 1910 is very frequently specified in Europe as a prior staining for complex after- 

 staining methods. During the early part of the present century, the substitution of ferric 

 chloride for ferric alum as a mordant was followed in many circles. Mordants other than iron 

 are rarely employed, although the introduction of copper by Faure 1924 has provided 

 histologists with a staining method considered by many people to be a definite improvement. 

 Diamond 1945 {Tech. Bull., 6:08) finds that the addition of .1% of a wetting agent (Tergitol 

 7) improves the stain. 



11.111 After Ferric Alum Mordants 



11.111 Benda 1893 2246, 7:161 



REAGENTS REQUIRED: A. ADS 12.1 Benda 1893 35, water 65; B. 1% hematoxylin; C. 



ADS 12.1 Benda 1893 5, water 95 

 method: [sections] —>■ water -^ A, 24 hrs. — > rinse -^ B, till black -+ C, till differentiated 

 — » balsam, via usual reagents 



11.111 Biitschli 1892 Butschli 1892, 80 



REAGENTS REQUIRED: A. 2% fernc acetate; B. 0.5% hematoxylin 



method: [sections of protozoans] — > A, 24 hrs. — > rinse — > B, 3 hrs. —>■ distilled water —> 

 balsam, via usual reagents 



11.111 Diamond 1945 519b, 15:68 



reagents required: A. 4% ferric alum; B. water 100, hematoxylin 0.5, Tergitol 7 0.1; 



C. sat. aq. sol. picric acid 

 method: [sections or smears] -^ water —> A, 5 mins. -^ rinse — » B, 5 mins. -^ rinse — > C, 

 till differentiated, 3-5 mins. —y tap water, till blue — > balsam, via carbol-xylene 



11.111 Dobell 1914 1798,34:139 



reagents required: A.1% ferric alum in 70% alcohol; B. 1% hematin in 70% alcohol; 



C. 0.1% HCI in 70% alcohol 

 method: [thin sections or protozoan smears]-^ 70% alcohol—* A, 10 mins. ^ B, 10 



mins. — > 70% alcohol, quick rinse —> C, till differentiated—* 70% alcohol, wash -^ 



counterstain — » balsam, via usual reagents 



11.111 Freitas 1936 1345, 31 :707 



reagents required: A. 0.5% ferric alum in 70% ale; B. 1% hematin in 70% ale. 



60 phosphate buffer pH 7.6 30; C. 0.3% picric acid 

 method: [sections] -^ A, 1 hr. -^ fi, 1 hr. -* wash -^ C, till differentiated -^ wash -» 

 balsam, via usual reagents 



11.111 French 1923 626,3:213 



REAGENTS REQUIRED: A. 3.5% ferric alum; B. 95% ale. 98, hematoxylin 1, sat. aq. sol. 



lithium carbonate 2; C. 1% ferric alum 

 method: [sections or smears] -^> yl, overnight -^ rinse —♦ B, overnight -^ wash —> C, 



till differentiated -^ wash 

 note: Solution B is attributed, without reference, to Rosenbush. 



11.111 Galiano 1928 see DS 13.6 Galiano 1928 



11.111 Haggquist 1933 23632, 60:77 



REAGENTS REQUIRED: A. 5% fcrric chloride; B. 1% hematoxylin; C. 1% ferric chloride. 

 method: [sections] -* water —» ^, 1 hr. —> quick rinse -^ B, 1 hr. — > wash — > C, till 

 differentiated — > wash -^ balsam, via usual reagents 



11.111 Hance 1933 19938,77:287 



STOCK FORMULA I: 10% hematoxylin in 95% alcohol 

 REAGENTS REQUIRED: A. 2.5% ferric alum; B. water 100 ml., stock I 10 ml., 2.5% 



sodium bicarbonate 0.1 

 method: [sections] -^ A, Yi to 2 hrs. -* distilled water, rinse — > /?, 3^ hr. — > tap water, 



rinse -^ .4, till differentiated — > tap water, till IjIuc -^ balsam, via usual reagents 



