282 METHODS AND FORMULAS DS 11.111-DS 11.112 



note: In preparing B solution, the bicarbonate should be added until the color changes 

 from yellow to plum. This color change is of more importance than the quantity of 

 alkali required to produce it. 



11.111 Heidenhain 1892 Feslchr. Kolliker, 118 



REAGENTS REQUIRED: A, 2.5% ferric alum; B. 0.5% hematoxylin, "ripened" at least on 



month 

 method: distilled water -^ A, 30 mins. to 24 hrs. -^ distilled water, rinse —* B, 30 mins. 



to 24 hrs. — > tap water, rinse, — > A, till differentiated—* tap water, till blue — » balsam, 



via usual reagents 

 note: Murray 1919 (1200, 16:77) substitutes 3.5% ferric alum for A, above. Masson 



1912 (4956, 87:291) substitutes 4% ferric alum for A and 1%, hematoxylin for B, 



mordanting and staining 5-10 minutes at 50°C. See also DS 12.31 Masson 1912. A 



detailed description of the use of this stain in the demonstration of chromosomes is 



given under DS 11.10. 



11.111 Hirsch and Bretschneider see DS 21.21 Hirsch and Bretschneider 1938 



11.111 Kofoid and Swegy 1915 16599, 51 :289 



reagents required: A. 0.4%, ferric alum in 50 %o ale; B. 0.50% hematoxylin in 70%, 



ale. 

 method: [smears] -> 50% ale. -^ A, 10 mins. -* rinse, 50% ale. — > B, 30 mins. -^ A, 



till differentiated —>■ wash thoroughly 

 recommended for: protozoan smears. 



11.111 Laudau see DS 21.212 Laudau 1924 



11.111 Loyez see DS 21.212 Loyez ,^ 



11.111 Markey, Culbertson, and Giordano 1943 see DS 23.33 Markey, et al. 1943 



11.111 Masson 1912 see DS 11.111 Heidenhain 1892 (note) 



11.111 Murray 1919 see DS 11.111 Heidenhain 1892 (note) 



11.111 Regaud 1910 1823, 11:291 



REAGENTS REQUIRED: A. 5% fcrric alum; B. water 80, hematoxylin 1, glycerol 10, 90% 



alcohol 10; C. ADS 21.1 Masson 1942 

 method: distilled water-* A, 30 mins., 50°C -^ distilled water, rinse -^ B, 30 mins., 

 50°C. -^ distilled water, wash -* C, till differentiated -^ tap water, till blue -^ bal- 

 sam, via usual reagents 

 note: a detailed description of the use of this stain before a complex contrast is given 

 under DS 12.30 below. 



11.111 Rosenbush see DS 11.111 French 1923 (note) 



11.111 Shortt 1923 9940, 10:836 



reagents required: A. 2.5% ferric alum; B. water 95, hematoxylin 1, phenol 5 

 method: identical with 11.111 Heidenhain 



11.112 After Ferric Chloride Mordants 



11.112 Cole 1926 19938, 64:452 

 stock solutions: abs. ale. 100, sodium hydrosulfite 1, hematoxylin 5 



reagents required: A. ADS 12.1 Cole 1926; B. Stock 3, ammonia 0.5, water 100; 

 C. 0.1%, hydrochloric acid; D. 0.01%, ammonia 



method: water -^ A, on slide, 5 mins. -* rinse -^ B, on slide, 10 mins. -^ C, till differ- 

 entiated — » D, till blue — > balsam, via usual reagents 



11.112 Mallory 1900 11189,5:18 



reagents required: A. 10% ferric chloride; B, 1% hematoxylin; C. Q.2b% ferric 

 chloride 



