DS 11.113 DYE STAINS OF GENERAL APPLICATION 283 



METiiou: [sections] > distilled water — > A, 3 5 niins. -^ li, on slide, draining and renew- 

 ing till precipitate ceases to form, till blue-black, 3-5 niins. — > tap water, wash 

 -^ C, till differentiated -^ tap water, wash — > balsam, via oil of cretan origanum 



11.113 After Other Mordants 



11.113 Apathy 1888 23632, 5:47 



REAGENTS REQUIRED: A. 1% hematoxylin; B. 1% potassium dichromate 

 method: [sections] —> water—* A, overnight-^ B, till stain first produced sufficiently 

 differentiated — > water, till yellow color removed -^ balsam, via usual reagents 



11.113 Bensley and Bensley 1938 Bensley and Bensley 1938, 79 



reagents required: A. sat. sol. copper acetate; B. 5% potassium chromate; C. 1% 



hematoxylin; D. water 80, ADS 21.1 Weigert 1885, 20 

 method: [sections] — > water -^ ^, 5 mins. -^ rinse — > B, dip — * rinse — > C, 2 mins. — > A, 

 1 min. — > D, till differentiated — * balsam, via usual reagents 



11.113 Drew 1920 see DS 22.0 Drew 1920 



11.113 Fajerstajn 1901 see DS 21.212 Fajerstajn 1901 



11.113 Heidenhain 1884 1780, 24:468 



reagents required: A. 0.3% hematoxylin; B. 0.5% potassium chromate 



method: [whole objects or sections]—* waters A, overnight^ rinse -^ B, till dark 



stain first produced sufficiently differentiated -^ water, till yellow color removed — » 



balsam, via usual reagents 



11.113 Knower 1930 19938, 72:172 



preparation of stock solution: Dissolve 5 hematoxylin in 100 abs. ale. Add 1 sat. sol. 

 sodium metabisulfite. 



REAGENTS REQUIRED: A. ADS 12.1 Colc 1916; B. stock 4, water 8, ammonia 1, 95% 

 alcohol 100; (Mix in order given; leave first three ingredients 30 seconds before 

 diluting); C. 0.1% hydrochloric acid; D. sat. sol. lithium carbonate in 70% alcohol 



method: [sections from material fixed in any copper-containing fixative (see Chapter 18, 

 F 1400, F 2400, F 3400, F 4000)] -^ 95% alcohol -^ A, 5 mins. -* 95% alcohol, quick 

 rinse -^ B, 5 mins. —> C, till differentiated -^ D, 2 mins. -^ balsam, via usual reagents 



note : Though this was originally intended as a nerve stain, it is far too good a general- 

 purpose stain to be omitted from this section. The original calls for "sodium bi- 

 sulphite," which is not soluble to the extent indicated; however "the bisulphite of 

 commerce usually consists chiefly, or almost entirely, of sodium pyrosulphite" (Merck 

 Index, 5th ed., 1940, 506). Sodium pyrosulfite, which is commonly sold as "metabi- 

 sulfite," is soluble to the required extent and is accordingly specified in the formula 

 given above. 



11.113 Kulschitzky 1889 766, 4:223 



formula: water 98, acetic acid 2, hematoxylin 1 



use: For general purposes as DS 11.113 Knower 1930. For nervous elements see Chap- 

 ter 21 DS 21.212. The formula of Welters 1890 (23632, 7:466) differs only in contain- 

 ing 2 parts hematoxylin. 



11.113 Mallory 1936 Lead hematoxylin see DS 21.212 Mallory 1936 



11.113 Nissl 1894 466, 51 :245 



reagents required: A. 2% ferric acetate in 90% ale; B. 1% hematoxylin in 70% ale.; 



C. 1% hydrochloric acid in 70% ale. 

 method: [sections of brain] —> A, 30 mins. — > rinses B, 30 mins. ^ rinse—* C, till 

 differentiated —> balsam, via usual reagents 



